Immunological assessment of mice hyperimmunized with native and cobalt-60-irradiated bothrops venoms
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Journal of Venomous Animals and Toxins Including Tropical Diseases
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ELISA was used to evaluate, accompany, and compare the humoral
immune response of Swiss mice during hyperimmunization with native and Cobalt60-irradiated (60Co) venoms of Bothrops jararaca, Bothrops jararacussu and
Bothrops moojeni. Potency and neutralization were evaluated by in vitro challenges.
After hyperimmunization, immunity was observed by in vivo challenge, and the side
effects were assessed. The animals immunization with one LD50 of each venom
occurred on days 1, 15, 21, 30, and 45, when blood samples were collected;
challenges happened on the 60th day. Results showed that ELISA was efficient in
evaluating, accompanying and comparing mouse immune response during
hyperimmunization. Serum titers produced with natural venom were similar to those
produced with irradiated venom. Immunogenic capacity was maintained after 60Coirradiation. The sera produced with native venom showed neutralizing potency and
capacity similar to those of the sera produced with irradiated venom. All antibodies
were able to neutralize five LD50 from these venoms. Clinical alterations were
minimum during hyperimmunization with irradiated venom, however, necrosis and
death occurred in animals inoculated with native venom.
Como referenciar
FERREIRA JUNIOR, R.S.; NASCIMENTO, N.; MARTINEZ, J.C.; ALVES, J.B.; MEIRA, D.A.; BARRAVIERA, B. Immunological assessment of mice hyperimmunized with native and cobalt-60-irradiated bothrops venoms. Journal of Venomous Animals and Toxins Including Tropical Diseases, v. 11, n. 4, p. 447-464, 2005. DOI: 10.1590/s1678-91992005000400006. Disponível em: http://repositorio.ipen.br/handle/123456789/8040. Acesso em: 20 Mar 2026.
Esta referência é gerada automaticamente de acordo com as normas do estilo IPEN/SP (ABNT NBR 6023) e recomenda-se uma verificação final e ajustes caso necessário.