Improvements in metal exposure assays
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International Journal of Environmental Research
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This technical note aims to report, in detail, the development, suitability and analytical validation of an artificial food to
assess metal bioaccumulation in the blue crab Callinectes danae after metal exposure assays. For this purpose, lead (Pb) was
chosen as the metal of interest. First, a non-contaminated artificial food was prepared by mixing commercial shrimps and
agarose. The food was then offered on alternate days to two groups of crabs for 7 and 14 days, respectively. After confirming
that this food was well-accepted by crabs, analytical procedures were performed to confirm that Pb added to the mixture
would remain in the artificial food. For this purpose, two foods containing Pb at concentrations of 0.5 and 2.0 μg g−
1 were
prepared by adding aliquots of an ultrapure Pb standard solution to the mixture. Samples of each food were subjected to
an acid extraction and Pb concentrations were determined by a GF-AAS. Pb recoveries in both contaminated foods were
> 90%. Subsequently, another experiment was performed, and two groups of crabs were fed with contaminated artificial
foods (0.5 and 2.0 μg g−
1 of Pb) during 7 and 14 days, respectively. The observed acceptance of these foods by crabs at both
Pb concentrations was of 100%.
Como referenciar
BORDON, ISABELLA C.; CAMPOS, BRUNO G. de; GUSSO‑CHOUERI, PALOMA K.; MIYAI, CAIO A.; ARAUJO, GIULIANA S. de; EMERENCIANO, ANDREWS K.; SILVA, JOSE R.M.C. da; COTRIM, MARYCEL E.B.; FAVARO, DEBORAH I.T.; ABESSA, DENIS M. de S. Improvements in metal exposure assays: artificial food to assess bioaccumulation in the blue crab Callinectes danae Smith, 1869 (Crustacea, Decapoda, Portunidae). International Journal of Environmental Research, v. 13, n. 2, p. 431-434, 2019. DOI: 10.1007/s41742-019-00170-3. Disponível em: http://repositorio.ipen.br/handle/123456789/30098. Acesso em: 20 Mar 2026.
Esta referência é gerada automaticamente de acordo com as normas do estilo IPEN/SP (ABNT NBR 6023) e recomenda-se uma verificação final e ajustes caso necessário.