Modification of gold nanoparticles with human serum albumin

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dc.contributor.authorSANTOS, C.S.F.
dc.contributor.authorSANTOS, J.J.
dc.contributor.authorLUGAO, A.B.
dc.contributor.editorBURITI, JOSUE da S.
dc.contributor.editorCRUZ, RITA de C.A.L.
dc.contributor.editorFOOK, MARCUS V.L.
dc.coverageNacionalpt_BR
dc.creator.eventoCONGRESSO DA SOCIEDADE LATINO AMERICANA DE BIOMATERIAIS, ORGÃOS ARTIFICIAIS E ENGENHARIA DE TECIDOS, 15.; CONGRESSO LATINO AMERICANO DE ÓRGÃOS ARTIFICIAIS E BIOMATERIAIS, 10.pt_BR
dc.date.accessioned2019-02-06T16:25:12Z
dc.date.available2019-02-06T16:25:12Z
dc.date.evento22 a 25 de agosto, 2018pt_BR
dc.description.abstractGold nanoparticles (AuNps) have been widely employed due to unique optical and electronic properties, and their excellent stability and low toxicity makes AuNps very interesting tool for be modified with biomolecules and applied on medicine. Albumin is one of the most important proteins present in blood, with the main function of transporting ions into the bloodstream. The AuNps preparation stabilized with human serum albumin (HSA), besides confer a great stability, makes a material with low body rejection, allowing transporting it into the cells. With this goal, this work aimed to finality to synthesize and characterize stabilized AuNps-HAS and subsequently be applied in the transport of radiopharmaceuticals. AuNps were synthesized by the Turkevich / Frens method, where 20 mg of tetrachlorouric acid, dissolved in 100 ml of water, are heated, and after boiling, 3 ml of sodium citrate (1% m:m) ). This method generates AuNps with 13 nm diameter, characterized by MET and UV-Vis spectroscopy analyzes show a presence of AuNps spectrum with absorption peak from 520nm due to the localized surface Plasmon resonances (LSPR). The citrate reduces gold and stabilizes the nanoparticles formed, and can be replaced by mercaptans such as 3-mercaptopropionic acid, a stabilizing agent capable of forming a self-assembled monolayer (SAM) that makes it possible to conjugate biomolecules on the surface of AuNps. This modification occurs after its synthesis and this occurs through the EDC / NHS coupling reagents, which activates the groups present in the reaction and increases the binding rate of the mercapto-acid carboxylic groups to the amine groups present in the albumin. In this way, a solution of HSA (at a final concentration of 0.25 mg / mL) and an EDC / NHS solution in a concentration of 1μM is added to the AuNps suspension. Confirmation of this modification occurs by the displacement of the LSPR band at lower energies (from 524 to 528 nm) and by means of dynamic light scattering measurements, increasing from 30 nm to 40 nm.pt_BR
dc.event.siglaSLABO; COLAOBpt_BR
dc.format.extent309-309pt_BR
dc.identifier.citationSANTOS, C.S.F.; SANTOS, J.J.; LUGAO, A.B. Modification of gold nanoparticles with human serum albumin. In: BURITI, JOSUE da S. (ed.); CRUZ, RITA de C.A.L. (ed.); FOOK, MARCUS V.L. (ed.). In: CONGRESSO DA SOCIEDADE LATINO AMERICANA DE BIOMATERIAIS, ORGÃOS ARTIFICIAIS E ENGENHARIA DE TECIDOS, 15.; CONGRESSO LATINO AMERICANO DE ÓRGÃOS ARTIFICIAIS E BIOMATERIAIS, 10., 22 a 25 de agosto, 2018, João Pessoa, PB. <b>Resumo...</b> Belo Horizonte, MG: Sociedade Latino Americana de Biomateriais, Engenharia de Tecidos e Órgãos Artificiais, 2018. p. 309-309. Disponível em: http://repositorio.ipen.br/handle/123456789/29498.
dc.identifier.orcidaguardandopt_BR
dc.identifier.orcidhttps://orcid.org/0000-0002-1737-3191
dc.identifier.urihttp://repositorio.ipen.br/handle/123456789/29498
dc.localBelo Horizonte, MGpt_BR
dc.local.eventoJoão Pessoa, PBpt_BR
dc.publisherSociedade Latino Americana de Biomateriais, Engenharia de Tecidos e Órgãos Artificiaispt_BR
dc.rightsopenAccesspt_BR
dc.subjectnanoparticles
dc.subjectgold
dc.subjectblood serum
dc.subjectalbumins
dc.subjectproteins
dc.subjectcitrates
dc.titleModification of gold nanoparticles with human serum albuminpt_BR
dc.typeResumo de eventos científicospt_BR
dspace.entity.typePublication
ipen.autorADEMAR BENEVOLO LUGAO
ipen.autorCAROLINA SILVA FERREIRA DOS SANTOS
ipen.codigoautor339
ipen.codigoautor14584
ipen.contributor.ipenauthorADEMAR BENEVOLO LUGAO
ipen.contributor.ipenauthorCAROLINA SILVA FERREIRA DOS SANTOS
ipen.date.recebimento19-02pt_BR
ipen.event.datapadronizada2018pt_BR
ipen.identifier.ipendoc25286pt_BR
ipen.notas.internasResumopt_BR
ipen.type.genreResumo
relation.isAuthorOfPublication99ac24c5-2ae1-465a-a6f2-40b4d9af6af7
relation.isAuthorOfPublication600a15d7-f100-43f9-af66-8dee344157eb
relation.isAuthorOfPublication.latestForDiscovery600a15d7-f100-43f9-af66-8dee344157eb
sigepi.autor.atividadeLUGAO, A.B.:339:740:Npt_BR
sigepi.autor.atividadeSANTOS, C.S.F.:14584:-1:S
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