International collaborative study to establish the 2nd WHO International Standard of Human Recombinant Fillicle Stimulating Hormone for Bioassai

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dc.contributor.authorOLIVEIRA, J.E.pt_BR
dc.contributor.authorALMEIDA, B.E.pt_BR
dc.contributor.authorDAMIANI, R.pt_BR
dc.contributor.authorBARTOLINI, P.pt_BR
dc.contributor.authorRIBELA, M.T.C.P.pt_BR
dc.coverageNacionalpt_BR
dc.creator.eventoREUNIAO ANUAL DA SOCIEDADE BRASILEIRA DE BIOQUIMICA E BIOLOGIA MOLECULAR, 39.pt_BR
dc.date.accessioned2014-11-19T11:35:04Zpt_BR
dc.date.accessioned2014-11-19T14:11:21Zpt_BR
dc.date.accessioned2015-04-01T14:10:20Z
dc.date.available2014-11-19T11:35:04Zpt_BR
dc.date.available2014-11-19T14:11:21Zpt_BR
dc.date.available2015-04-01T14:10:20Z
dc.date.evento18-21 de maio, 2010pt_BR
dc.description.abstractThe aim of the International Collaborative Study proposed by the National Institute for Biological Standards and Control (WHO) is to calibrate a new preparation of human follicle stimulating hormone (hFSH) relative to the 1st International Standard for bioassay of recombinant-hFSH (WHO 92/642), which is almost exhausted and requires to be replaced. The candidate preparation and four other samples, which correspond to this preparation stored at elevated temperatures (K, L, M, N), were analyzed qualitatively and quantitatively in our laboratory, by reversed-phase high-performance liquid chromatography (RPHPLC). The candidate content determined in six assays (8.59 ± 0.41 μg/ampoule) was 20% lower, when compared with the current standard. Based on this, an average value of 110 IU FSH per ampoule could be assigned to this preparation. The main isoform of the candidate preparation showed a lower retention time (22.5 ± 0.83 min) relative to WHO 92/642 (22.8 ± 0.81 min) and also to an internal reference preparation (22.6 ± 0.86 min). These differences, though, were not significant (p>0.05). Practically identical contents were also determined for K, L, M and N ampoules (8.44 ± 0.20; 8.56 ± 0.33; 8.57 ± 0.38; 8.69 ± 0.30 μg/ampoule, respectively). No significant differences in FSH retention times between the candidate preparation and the ampoules stored at elevated temperatures were observed.
dc.event.siglaSBBq 2010pt_BR
dc.format.extent71pt_BR
dc.identifier.citationOLIVEIRA, J.E.; ALMEIDA, B.E.; DAMIANI, R.; BARTOLINI, P.; RIBELA, M.T.C.P. International collaborative study to establish the 2nd WHO International Standard of Human Recombinant Fillicle Stimulating Hormone for Bioassai. In: REUNIAO ANUAL DA SOCIEDADE BRASILEIRA DE BIOQUIMICA E BIOLOGIA MOLECULAR, 39., 18-21 de maio, 2010, Foz do Iguacu, PR. <b>Resumos...</b> p. 71. Disponível em: http://repositorio.ipen.br/handle/123456789/21256.
dc.identifier.urihttp://repositorio.ipen.br/handle/123456789/21256pt_BR
dc.local.eventoFoz do Iguacu, PRpt_BR
dc.rightsopenAccesspt_BR
dc.subjectfshpt_BR
dc.subjecthigh-performance liquid chromatographypt_BR
dc.subjectgene recombinationpt_BR
dc.subjecthormonespt_BR
dc.subjectbioassaypt_BR
dc.subjectinternational regulationspt_BR
dc.subjectcalibrationpt_BR
dc.subjectcomparative evaluationspt_BR
dc.titleInternational collaborative study to establish the 2nd WHO International Standard of Human Recombinant Fillicle Stimulating Hormone for Bioassaipt_BR
dc.typeResumo de eventos científicospt_BR
dspace.entity.typePublication
ipen.autorPAOLO BARTOLINI
ipen.autorMARIA TERESA DE CARVALHO PINTO RIBELA
ipen.autorRENATA DAMIANI
ipen.autorBEATRIZ ELANE DE ALMEIDA
ipen.autorJOAO EZEQUIEL DE OLIVEIRA
ipen.codigoautor1503
ipen.codigoautor1197
ipen.codigoautor3931
ipen.codigoautor6043
ipen.codigoautor425
ipen.contributor.ipenauthorPAOLO BARTOLINI
ipen.contributor.ipenauthorMARIA TERESA DE CARVALHO PINTO RIBELA
ipen.contributor.ipenauthorRENATA DAMIANI
ipen.contributor.ipenauthorBEATRIZ ELANE DE ALMEIDA
ipen.contributor.ipenauthorJOAO EZEQUIEL DE OLIVEIRA
ipen.date.recebimento11-01pt_BR
ipen.event.datapadronizada2010pt_BR
ipen.identifier.ipendoc16159pt_BR
ipen.notas.internasResumospt_BR
ipen.type.genreResumo
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relation.isAuthorOfPublicatione9e6346c-9b8b-4d39-8bdd-505ea6997646
relation.isAuthorOfPublication.latestForDiscoverye9e6346c-9b8b-4d39-8bdd-505ea6997646
sigepi.autor.atividadeOLIVEIRA, J.E.:425:810:Spt_BR
sigepi.autor.atividadeALMEIDA, B.E.:6043:810:Npt_BR
sigepi.autor.atividadeDAMIANI, R.:3931:810:Npt_BR
sigepi.autor.atividadeBARTOLINI, P.:1503:810:Npt_BR
sigepi.autor.atividadeRIBELA, M.T.C.P.:1197:810:Npt_BR
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