Development of a microfluidic circuit with femtosecond laser technology for enzyme-linked immunosorbent assay validation
Carregando...
Data
Data de publicação
2017
Orientador
Título da Revista
ISSN da Revista
Título do Volume
É parte de
É parte de
É parte de
É parte de
WORKSHOP EM MICROFLUÍDICA, 7.
Exportar
Resumo
One of the great challenges in science today is to develop applications for disease diagnosis that can be accessible to the poorest populations distant from large centers. Using ultra-short laser pulses, we developed and machined a microfluidic circuit capable of replicating enzyme-linked immunosorbent assays (ELISA) with a much lower cost of materials. Microchannels etched in BK7 glass separated by a PDMS film form the basis of the system. The reagent flow control is done by pneumatic micro-valves controlled by an Arduino microcontroller through a Labview platform. The enzymatic reactions occurred under stopped-flow conditions, in a portion of the circuit constructed in a serpentine shape to facilitate the detection by an image processing software. This work presents the design of the microfluidic circuit developed and an ELISA test for characterization and validation of the results.
Como referenciar
HERRERA, CRISTHIANO C.; RIBEIRO, MARTHA S.; SPENCER, PATRICK J.; SAMAD, RICARDO E.; VIEIRA JUNIOR, NILSON D.; ROSSI, WAGNER de. Development of a microfluidic circuit with femtosecond laser technology for enzyme-linked immunosorbent assay validation. In: WORKSHOP EM MICROFLUÍDICA, 7., 31 de julho - 3 de agosto, 2017, São Paulo, SP. Abstract... Disponível em: http://repositorio.ipen.br/handle/123456789/28082. Acesso em: 24 Mar 2026.
Esta referência é gerada automaticamente de acordo com as normas do estilo IPEN/SP (ABNT NBR 6023) e recomenda-se uma verificação final e ajustes caso necessário.