Chitosan hydrogel containing bioactive glass aiming at 3D printing for peripheralnerve regeneration
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2023
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LATIN-AMERICAN CONGRESS OF ARTIFICIAL ORGANS AND BIOMATERIALS, 12th
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Introduction and objective: Peripheral nerve lesions are a challenge in the context of regeneration. Tissue
engineering strategy can increase repair success using biomaterials and cells. Chitosan is a biocompatible
natural polymer, with good cost-effectiveness and rheological properties that has been shown to be
suitable for nerve regeneration. Bioactive glasses exhibited the ability to increase the production of
neurotrophicsubstances in in vivo experiments that could assist nerve repair. 3D printing presents the
possibility of building complex and patient-specific structures. Thus, the development of chitosan
hydrogels with different concentrations of bioactive glasses for 3D printing is proposed for future
application in peripheral nerveregeneration.
Methodology: Chitosan hydrogel was produced with different concentrations of bioactive glass (0, 0.5%,
1%, 2%, and 5%). The rheological characterization of the hydrogels was carried out by shear sweep and
creep- recovery experiments. Printability and shape fidelity were evaluated after the 3D printing process
through the comparison of the theoretical CAD model and printed structure. Indirect cytotoxicity assay
using PC12 cells wasperformed to evaluate the biological properties of the scaffolds.
Results and discussion: The presence of glass affects the rheological properties of chitosan hydrogel. The
shear sweep results showed that the viscosity of the chitosan containing bioactive glass decreases at the
lowest shearrate compared to pure chitosan hydrogel. However, the viscosity of hydrogels containing
higher concentrations of glass (2% and 5%) increases when compared to the other composites. Such
behaviour is a consequence of two events: the presence of the bioactive glass particles and the ions
release, caused by glass dissolution in the material, which can interact with the polymeric chains and
disrupt the formation of intermolecular interactions. Furthermore, the incorporation of the ceramic
particles increased the recovery percentage of the material. This raise is caused by the interactions
between the bioactive glass surface and chitosan chains, increasing the hydrogel’s stability. These
rheological characteristics improved shape fidelity of the material and possible use as ink for 3D printing.
Biologically, the extracts of the materials had a non-toxic effect on the PC12 cells.
Conclusions: The presence of glass affects the rheological properties of chitosan hydrogel to improve its
use as 3D printing ink. The biological experiments showed that the materials do not have cytotoxic effects
and could be used in tissue engineering. Therefore, chitosan hydrogel with bioactive glass particles are
promising materials for application in peripheral nerve regeneration.
Como referenciar
LOURENÇO, LARISSA; BORGES, ROGER; CARASTAN, DANILO; SILVA, CASTRO; MATHOR, MONICA B.; MARCHI, JULIANA. Chitosan hydrogel containing bioactive glass aiming at 3D printing for peripheralnerve regeneration. In: LATIN-AMERICAN CONGRESS OF ARTIFICIAL ORGANS AND BIOMATERIALS, 12th, December 12-15, 2023, Mar del Plata, Argentina. Abstract... p. 133-133. Disponível em: https://repositorio.ipen.br/handle/123456789/47940. Acesso em: 30 Dec 2025.
Esta referência é gerada automaticamente de acordo com as normas do estilo IPEN/SP (ABNT NBR 6023) e recomenda-se uma verificação final e ajustes caso necessário.