Radiation-synthesized protein-based drug carriers: Size-controlled BSA nanoparticles
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International Journal of Biological Macromolecules
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Nanotechnology has broadened the options for the delivery of agents of biotechnological and clinical relevance. Currently, attention has been driven towards the development of protein-based nanocarriers due to high the biocompatibility and site-specific delivery. In this work we report radiation-synthesized bovine serum albumin nanoparticles as an attempt to overcome limitations of available albumin particles, as a novel route for the development of crosslinked protein nanocarriers for the administration of chemotherapic agents or radiopharmaceuticals. Albumin containing phosphate buffer solutions were irradiated using γ-irradiation at distinct cosolvent concentrations—ethanol or methanol. Nanoparticle size was followed by DLS and bityrosine crosslinking formation using fluorescence measurements and SDS-PAGE. In addition, computational experiments were performed to elucidate the mechanism and pathways for the nanoparticle formation. The synthesis of BSA nanoparticles using γ-irradiation in the presence of a cosolvent allowed the formation of the nanoparticles from 7 to 70 nm without the use of any chemical crosslinker as confirmed by SDS-PAGE and DLS analysis. The combination of cosolvent and γ-irradiation allowed a fine tuning with regard to protein size.
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QUEIROZ, R.G.; VARCA, G.H.C.; KADLUBOWSKI, S.; ULANSKI, P.; LUGAO, A.B. Radiation-synthesized protein-based drug carriers: Size-controlled BSA nanoparticles. International Journal of Biological Macromolecules, v. 85, p. 82-91, 2016. Disponível em: http://repositorio.ipen.br/handle/123456789/25816. Acesso em: 30 Jun 2026.
Esta referência é gerada automaticamente de acordo com as normas do estilo IPEN/SP (ABNT NBR 6023) e recomenda-se uma verificação final e ajustes caso necessário.