Zinc and manganese imbalances in BALB/c mice experimentally infected with Leishmania (Leishmania) amazonensis
Carregando...
Data
Data de publicação
Orientador
Título da Revista
ISSN da Revista
Título do Volume
É parte de
É parte de
É parte de
Acta Parasitologica
Exportar
Resumo
Abstract
Purpose The clinical progression of Leishmania (Leishmania) amazonensis infection depends on multiple factors, including immunological status of the host and their genotypic interaction. Several immunological processes depend directly on
minerals for an efcient performance. Therefore, this study used an experimental model to investigate the alterations of
trace metals in L. amazonensis infection associate with clinical outcome, parasite load, and histopathological lesions, and
the efect of CD4+T cells depletion on these parameters.
Methods A total of 28 BALB/c mice were divided into 4 groups: 1—non-infected; 2—treated with anti-CD4 antibody; 3—
infected with L. amazonensis; and 4—treated with anti-CD4 antibody and infected with L. amazonensis. After 24 weeks postinfection, levels of calcium (Ca), iron (Fe), magnesium (Mg), manganese (Mn), Cu, and Zn were determined by inductively
coupled plasma optical emission spectroscopy using tissue samples of the spleen, liver, and kidneys. Additionally, parasite
burdens were determined in the infected footpad (inoculation site) and samples of inguinal lymph node, spleen, liver, and
kidneys were submitted to histopathological analysis.
Results Despite no signifcant diference was observed between groups 3 and 4, L. amazonensis-infected mice had a signifcant reduction of Zn (65.68–68.32%) and Mn (65.98 to 82.17%) levels. Presence of L. amazonensis amastigotes was also
detected in the inguinal lymph node, spleen, and liver samples in all infected animals.
Conclusion The results showed that signifcant alterations in micro-elements levels occur in BALB/c mice experimentally
infected with L. amazonensis and may increase the susceptibility of individuals to the infection.
Como referenciar
SOBOTYK, CAROLINE; BALDISSERA, FERNANDA G.; RODRIGUES JUNIOR, LUIZ C.; ROMAO, PEDRO R.T.; OLIVEIRA, JULIANA S. de; DORNELLES, GUILHERME L.; ANDRADE, CINTHIA M. de; MACIEL, ROBERTO M.; DANESI, CRISTIANE C.; FERREIRA, RAFAEL V. de P.; BELLINI, MARIA H.; BOTTON, SONIA de A.; VOGEL, FERNANDA S.F.; SANGIONI, LUIS A. Zinc and manganese imbalances in BALB/c mice experimentally infected with Leishmania (Leishmania) amazonensis. Acta Parasitologica, v. 68, n. 2, p. 447-452, 2023. DOI: 10.1007/s11686-023-00666-1. Disponível em: http://repositorio.ipen.br/handle/123456789/34091. Acesso em: 30 Dec 2025.
Esta referência é gerada automaticamente de acordo com as normas do estilo IPEN/SP (ABNT NBR 6023) e recomenda-se uma verificação final e ajustes caso necessário.