Photodynamic action of toluidine blue in streptococcus mutans by fluorescence spectroscopy

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dc.contributor.authorNUNEZ, S.C.pt_BR
dc.contributor.authorGOMES, L.pt_BR
dc.contributor.authorGARCEZ, A.S.pt_BR
dc.contributor.authorMULLER, K.P.pt_BR
dc.contributor.authorJORGE, A.O.C.pt_BR
dc.contributor.authorRIBEIRO, M.S.pt_BR
dc.coverageInternacionalpt_BR
dc.date.accessioned2022-02-21T18:45:20Z
dc.date.available2022-02-21T18:45:20Z
dc.date.issued2004pt_BR
dc.description.abstractThe antimicrobial activity of toluidine blue associated with red light has been demonstrated for a wide range of microorganisms including those commonly found in infected root canals, carious lesions and periodontal pockets. Recent reports have drawn attention to the problems of antimicrobial resistance and resistance of oral bacteria to antibiotics and local antiseptics is of increasing concern, thus photodynamic therapy could be an alternative antimicrobial approach to treat localized infections in oral cavity. In this study the fluorescence spectra of TB were obtained before and after laser exposure in the presence or absence of Streptococcus mutans. The dye concentration was 0.01%, the irradiation was performed with a diode laser, l= 660 nm, P= 40mW, exposure time of 3 minutes in a volume of 0.5 ml, with a pre-irradiation time (PIT) of one or five minutes. The results showed shifts in fluorescence spectra observed for different preirradiation times in the presence of S. mutans. In the absence of bacteria, a shift in the spectra was observed in the dye before and after irradiation. These findings may indicate a photobleaching of the dye denoting structural alterations after irradiation and confirm the importance of the PIT for the success of this therapy.pt_BR
dc.format.extent84-85pt_BR
dc.identifier.citationNUNEZ, S.C.; GOMES, L.; GARCEZ, A.S.; MULLER, K.P.; JORGE, A.O.C.; RIBEIRO, M.S. Photodynamic action of toluidine blue in streptococcus mutans by fluorescence spectroscopy. <b>Brazilian Dental Journal</b>, v. 15, p. 84-85, 2004. Special issue. Disponível em: http://repositorio.ipen.br/handle/123456789/32756.
dc.identifier.issn0103-6440pt_BR
dc.identifier.orcid0000-0002-4203-1134pt_BR
dc.identifier.orcid0000-0003-2101-8333pt_BR
dc.identifier.orcidhttps://orcid.org/0000-0002-4203-1134
dc.identifier.percentilfiSem Percentilpt_BR
dc.identifier.percentilfiCiteScoreSem Percentil CiteScorept_BR
dc.identifier.suplementoSpecial issuept_BR
dc.identifier.urihttp://repositorio.ipen.br/handle/123456789/32756
dc.identifier.vol15pt_BR
dc.relation.ispartofBrazilian Dental Journalpt_BR
dc.rightsopenAccesspt_BR
dc.sourceInternational Congress on Laser in Dentistry, 9th, July 21-24, 2004, São Paulo, SPpt_BR
dc.subjectdentistry
dc.subjectcaries
dc.subjectbacteria
dc.subjectantimicrobial agents
dc.subjecttoluidine blue
dc.titlePhotodynamic action of toluidine blue in streptococcus mutans by fluorescence spectroscopypt_BR
dc.typeResumos em periódicospt_BR
dspace.entity.typePublication
ipen.autorMARTHA SIMOES RIBEIRO
ipen.autorKARIN PRAIA MULLER
ipen.autorSILVIA CRISTINA NUNEZ
ipen.codigoautor574
ipen.codigoautor3403
ipen.codigoautor3759
ipen.contributor.ipenauthorMARTHA SIMOES RIBEIRO
ipen.contributor.ipenauthorKARIN PRAIA MULLER
ipen.contributor.ipenauthorSILVIA CRISTINA NUNEZ
ipen.date.recebimento22-02
ipen.identifier.fiSem F.I.pt_BR
ipen.identifier.fiCiteScoreSem CiteScorept_BR
ipen.identifier.ipendoc12376pt_BR
ipen.type.genreResumo
relation.isAuthorOfPublication36215a53-0150-4910-91d7-9559717b62d7
relation.isAuthorOfPublicationa73f9bb4-73ff-4a2e-a711-bf8957ad0f6c
relation.isAuthorOfPublication8e1301a8-69c4-4aa3-a893-5a23b5328a83
relation.isAuthorOfPublication.latestForDiscovery8e1301a8-69c4-4aa3-a893-5a23b5328a83
sigepi.autor.atividadeRIBEIRO, M.S.:574:920:Npt_BR
sigepi.autor.atividadeMULLER, K.P.:3403:-1:Npt_BR
sigepi.autor.atividadeNUNEZ, S.C.:3759:920:Spt_BR
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