Photodynamic action of toluidine blue in streptococcus mutans by fluorescence spectroscopy
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2004
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Brazilian Dental Journal
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Resumo
The antimicrobial activity of toluidine blue associated with red
light has been demonstrated for a wide range of microorganisms
including those commonly found in infected root canals, carious lesions and periodontal pockets. Recent reports have drawn
attention to the problems of antimicrobial resistance and resistance
of oral bacteria to antibiotics and local antiseptics is of
increasing concern, thus photodynamic therapy could be an alternative
antimicrobial approach to treat localized infections in oral
cavity. In this study the fluorescence spectra of TB were obtained
before and after laser exposure in the presence or absence of
Streptococcus mutans. The dye concentration was 0.01%, the
irradiation was performed with a diode laser, l= 660 nm, P=
40mW, exposure time of 3 minutes in a volume of 0.5 ml, with a
pre-irradiation time (PIT) of one or five minutes. The results
showed shifts in fluorescence spectra observed for different preirradiation
times in the presence of S. mutans. In the absence of
bacteria, a shift in the spectra was observed in the dye before and
after irradiation. These findings may indicate a photobleaching
of the dye denoting structural alterations after irradiation and
confirm the importance of the PIT for the success of this therapy.
Como referenciar
NUNEZ, S.C.; GOMES, L.; GARCEZ, A.S.; MULLER, K.P.; JORGE, A.O.C.; RIBEIRO, M.S. Photodynamic action of toluidine blue in streptococcus mutans by fluorescence spectroscopy. Brazilian Dental Journal, v. 15, p. 84-85, 2004. Special issue. Disponível em: http://repositorio.ipen.br/handle/123456789/32756. Acesso em: 06 Mar 2025.
Esta referência é gerada automaticamente de acordo com as normas do estilo IPEN/SP (ABNT NBR 6023) e recomenda-se uma verificação final e ajustes caso necessário.