KARIN PRAIA MULLER
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Resumo IPEN-doc 14036 Local Treatment using high and low-power laser on herpes simplex2004 - MULLER, K.P.; YAMADA JUNIOR, A.M.; SUGAYAMA, S.T.; RIBEIRO, M.S.Herpes simplex (HSV) is commonly encountered in the dentistry practice disappearing after 10 to 14 days. Recently alternative treatments have been introduced, among those laser therapy. The aim of this study is to evaluate the efficacy of laser therapy in patients with perioral herpes simplex infection. A 32 years old female was treated with high-intensity laser therapy (HILT) in vesicle phase using a Nd:YAG laser, l= 1064 nm, P= 1,5 W, f= 15 Hz on days 1 and 2 to drainage the vesicles. Low-intensity laser therapy (LILT) was applied on the affected area in the 3 consecutive days with a GaAlAs laser, l= 830 nm, D= 5 J/cm2. On the other hand, a 7 years male patient, which presented the vesiculo-ulcerative stage, only LILT (l= 660 nm, D= 4 J/cm2) was carried out. For both cases, it was observed that the course of the infection was quicker than the normal. Seven days after treatment beginning the lesions were healed. These findings suggest that LILT associated or not to HILT is a efficient and promising treatment for HSV. Further larger studies aiming at confirming these data and investigating the LILT mechanisms of action should be performed.Resumo IPEN-doc 12376 Photodynamic action of toluidine blue in streptococcus mutans by fluorescence spectroscopy2004 - NUNEZ, S.C.; GOMES, L.; GARCEZ, A.S.; MULLER, K.P.; JORGE, A.O.C.; RIBEIRO, M.S.The antimicrobial activity of toluidine blue associated with red light has been demonstrated for a wide range of microorganisms including those commonly found in infected root canals, carious lesions and periodontal pockets. Recent reports have drawn attention to the problems of antimicrobial resistance and resistance of oral bacteria to antibiotics and local antiseptics is of increasing concern, thus photodynamic therapy could be an alternative antimicrobial approach to treat localized infections in oral cavity. In this study the fluorescence spectra of TB were obtained before and after laser exposure in the presence or absence of Streptococcus mutans. The dye concentration was 0.01%, the irradiation was performed with a diode laser, l= 660 nm, P= 40mW, exposure time of 3 minutes in a volume of 0.5 ml, with a pre-irradiation time (PIT) of one or five minutes. The results showed shifts in fluorescence spectra observed for different preirradiation times in the presence of S. mutans. In the absence of bacteria, a shift in the spectra was observed in the dye before and after irradiation. These findings may indicate a photobleaching of the dye denoting structural alterations after irradiation and confirm the importance of the PIT for the success of this therapy.Dissertação IPEN-doc 10285 Artigo IPEN-doc 11901 Effects of low power red laser on induced-dental caries in rats2007 - MUELLER, KARIN P.; RODRIGUES, CELIA R.M.D.; NUNEZ, SILVIA C.; ROCHA, RAQUEL; JORGE, ANTONIO O.C.; RIBEIRO, MARTHA S.Objective: The purpose of this study was to investigate the effects of low power red laser associated with acidulated phosphate fluoride on the development of induced-dental caries in rats. Design: Dental caries were induced in molars of 40 rats divided into five groups: control group (CG), the teeth were not submitted to any treatment; laser group (LG), teeth were irradiated with a low power red laser (LPRL), power of 30 mW and dose of 5 J/cm2; fluoride group (FG), teeth were treated with topical acidulated phosphate fluoride (APF) 1.23% applied for 4 min; laser + fluoride group (LFG), teeth were irradiated with LPRL followed by APF; fluoride + laser group (FLG), teeth were treated with APF followed by LPRL. The animals were killed after 48 days, and the first and second molars were extracted to analyze the caries lesion area, microhardness, and calcium and phosphorus ratio. Results: There were no statistical differences among FG, LFG, and FLG regarding to caries area and microhardness, although the caries area were smaller in LFG. Ca/P ratio did not show significant differences among all groups. Conclusions: Although LPRL before APF application appeared to diminish the caries progression, LPRL did not present any additional benefit compared with acidulated phosphate fluoride on the prevention of induced-dental caries in rats.