MARTHA SIMOES RIBEIRO

Resumo

Martha S. Ribeiro possui Graduação em Física pela Universidade Estadual de Campinas (1987), Mestrado em Ciências pelo Instituto de Pesquisas Energéticas e Nucleares (1991) e Doutorado em Ciências pelo Instituto de Pesquisas Energéticas e Nucleares (2000). Atualmente é pesquisadora titular do IPEN-CNEN, vice-coordenadora da Comissão de Graduação do Ipen, membro da Comissão PIBIC, membro do Núcleo de Avaliação de Tecnologias em Saúde (NATS-IPEN) e docente permanente do programa de pós graduação em Tecnologia Nuclear da USP e em Tecnologia das Radiações nas Ciências da Saúde do IPEN. Foi pesquisadora visitante na Università Tor Vergata (Roma, Itália), Universiteit van Amsterdam (Holanda) e na Université Catolique Du Louvain (Louvain-la-neuve, Bélgica). Faz parte da diretoria da Academia Brasileira de Biofotônica e Laser e é membro titular da Sociedade Brasileira de Física, Sociedade Brasileira de Biofísica, International Photodynamic Association e da Sociedade Internacional para Óptica e Fotônica (SPIE). Já formou mais de 50 pós-graduandos, entre mestres e doutores. Tem colaboração ativa com pesquisadores nacionais e internacionais e já publicou mais de 150 trabalhos em revistas científicas, com mais de 3000 citações. Publicou 4 livros em parceria com profissionais da área para disseminar o uso de tecnologias baseadas em luz na clínica odontológica e veterinária. É parecerista de artigos científicos para revistas nacionais e internacionais. É assessora ad hoc para agências de fomento no Brasil e exterior. Tem projetos em colaboração com empresas e hospitais visando a transferência de tecnologias baseadas em luz em prol da população. Ministra palestras e cursos sobre tecnologias baseadas em luz para pesquisadores e profissionais da Saúde. Atua no desenvolvimento de processos não térmicos baseados e/ou assistidos por luz para terapia e diagnóstico. Seus interesses envolvem biofotônica, tecnologia das radiações na saúde, óptica biomédica, nanomateriais e engenharia de tecidos. (Texto extraído do Currículo Lattes em 17 nov. 2021)

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Agora exibindo 1 - 10 de 396
  • Resumo IPEN-doc 30487
    Porphyrin-coated gold nanoparticles associated with ionizing radiation in the treatment of triple-negative breast cancer
    2024 - SILVA, CAMILA R.; KAYANI, ZAHRA; RIBEIRO, MARTHA S.; BERIONNI, GUILLAUME; HEUSKIN, ANNE-CATHERINE
    Triple-negative breast cancer (TNBC) is a subtype of the breast cancer that represents around 20% of all invasive breast cancer, which main characteristics is resistance to conventional treatments, such as exposure to ionizing radiation (IR). On the order hand, photodynamic therapy (PDT) using porphyrins has been described in the literature as a potential therapy against cancer and currently with the advance of nanomedicine, nanoparticles (NPs) have been used to deliver the photosensitizer with greater precision mainly in deep tumor. Thus, our goal in this work was to develop a NP coated with TMPyP and associate it with IR in the treatment of TNBC.
  • Artigo IPEN-doc 29702
    Radioactive seed localization for nonpalpable breast lesions
    2024 - FERREIRA, HORTENCIA H.J.; SOUZA, CARLA D. de; POZZO, LORENA; RIBEIRO, MARTHA S.; ROSTELATO, MARIA E.C.M.
    Background: This study is a systematic review with meta-analysis comparing radioactive seed localization (RSL) versus radio-guided occult lesion localization (ROLL) and wire-guided localization (WGL) for patients with impalpable breast cancer undergoing breast-conserving surgery and evaluating efficacy, safety, and logistical outcomes. The protocol is registered in PROSPERO with the number CRD42022299726. Methods: A search was conducted in the Embase, Lilacs, Pubmed, Scielo, Web of Science, and clinicaltrials.gov databases, in addition to a manual search in the reference list of relevant articles, for randomized clinical trials and cohort studies. Studies selected were submitted to their own data extraction forms and risk of bias analysis according to the ROB 2 and ROBINS 1 tools. A meta-analysis was performed, considering the random effect model, calculating the relative risk or the mean difference for dichotomous or continuous data, respectively. The quality of the evidence generated was analyzed by outcome according to the GRADE tool. Overall, 46 articles met the inclusion criteria and were included in this systematic review; of these, 4 studies compared RSL and ROLL with a population of 1550 women, and 43 compared RSL and WGL with a population of 19,820 women. Results: The results showed that RSL is a superior method to WGL in terms of surgical efficiency in the impalpable breast lesions’ intraoperative localization, and it is at least equivalent to ROLL. Regarding security, RSL obtained results equivalent to the already established technique, the WGL. In addition to presenting promising results, RSL has been proven to be superior to WGL and ROLL technologies.
  • Resumo IPEN-doc 30246
    Biotribological characterization of laser textured Ti6Al4V produced by addictive manufacturing
    2023 - PIERETTI, E.F.; OLIVEIRA, M.C.L.; ANTUNES, R.A.; RIBEIRO, M.S.; NEVES, M.D.M.
  • Resumo IPEN-doc 30133
    Photobiomodulation therapy combined with radioactive gold nanoparticles in breast cancer-bearing mice
    2023 - PINTO, MAYARA S.; ROSERO, WILMMER A.A.; ROSTELATO, MARIA E.M.C.; RIBEIRO, MARTHA S.
    Breast cancers are responsible for the highest number of worldwide deaths among women requiring new therapeutic strategies to promote better life’s quality and survival rates for patients. Nanobrachytherapy (NB) and photobiomodulation therapy (PBM) emerge in this context as less invasive approaches for breast cancer treatment. NB is a technique that might deliver high doses of ionizing radiation in a shorter time and in a localized way in comparison to conventional radiotherapy and PBM could enhance cell sensitivity when applied before NB. In this work, we aim to investigate the impact of PBM combined with NB involving radioactive gold nanoparticles (AuNPs) in the treatment of breast cancer in a murine model. Murine 4T1 cells were cultivated in RPMI medium and after injected into the lower-left mammary fat pad animals’ breast. When the tumor reached approximately 0.1 cm3, AuNPs (~284 microCi) were inoculated in the mouse breast. Thereafter, the tumor was irradiated with a red LED (660 nm, 40 mW, 150 s, 6 J) in a single application. Animals were monitored for 3 weeks until euthanasia. Our results demonstrated an arrestment in tumor growth for NB group in comparison to Control, PBM and PBM + NB groups. These data suggest that a single PBM session was not able to enhance the NB of breast cancer. The following steps will involve applying NB and PBM in two sessions. The first session will take place on the same day as NB application, and the second one after 6 days, corresponding to two half-lives of 198AuNPs.
  • Resumo IPEN-doc 30132
    Photodynamic inactivation against the critical priority pathogen Candida auris
    2023 - SILVA, ABDENEGO R.; CABRAL, FERNANDA V.; SILVA, CAMILA R.; SILVA, DANIELA F.T.; FREITAS, ANDERSON Z.; FONTES, ADRIANA; RIBEIRO, MARTHA S.
    Fungal pathogens and their associated infections present a growing challenge to global public health. Among these pathogens, Candida auris has emerged as a highly hazardous hospital-acquired microorganism, included in the critical priority group by the World Health Organization. Methylene Blue (MB) is a widely acknowledged photosensitizer utilized in antifungal photodynamic inactivation (PDI) and holds significant clinical applications. The MB methylation results in the formation of a more lipophilic compound, the 1,9-dimethyl MB (DMMB), which can have an enhanced interaction with cell membranes. Nevertheless, PDI mediated by DMMB to combat fungi remains little explored. In this study, we assessed the impact and underlying mechanisms of PDI using MB (MB-PDI) or DMMB (DMMB-PDI) combined with a red LED against C. auris. PDI was conducted on the CBS 10913 strain of C. auris, utilizing different concentrations of MB (0 – 100 μM) or DMMB (0 – 3 μM) at light doses of 10 or 30 J/cm². To evaluate the PDI efficacy, we measured colony-forming units and monitored reactive oxygen species (ROS) production. Additionally, we assessed lipid peroxidation (LPO) and mitochondrial membrane potential (ΔΨm) to gain insights into the differences between MB and DMMB. Our findings revealed that DMMB-PDI successfully eradicated C. auris yeasts at 3 μM concentration, irrespective of the light dose, whereas MB (100 μM) only exhibited cell eradication at the highest light dose. ROS formation was more pronounced for DMMB than MB at 10 J/cm2. At 30 J/cm2, MB and DMMB produced similar ROS levels. In sublethal conditions, DMMB-PDI induced significantly higher LPO, and ΔΨm levels compared to MB-PDI. Furthermore, DMMB-PDI effectively inhibited biofilm formation and disrupted mature biofilms, with no observed toxicity in fibroblast cells. In conclusion, our study demonstrates the potential of DMMB-PDI as a promising weapon to combat the global priority pathogen C. auris. The enhanced PDI efficacy and biofilm eradication capacity of DMMB make it a valuable candidate for further exploration in the fight against this hazardous pathogen. As the incidence of drug-resistant fungal infections continues to rise, the development of innovative and effective therapeutic strategies like DMMB-PDI is crucial in safeguarding public health worldwide.
  • Resumo IPEN-doc 30131
    Photodynamic inactivation of Candida glabrata boosted by association of silver nanoparticles with cationic ZnTE-2-PyP4+ porphyrin
    2023 - LIMA, GEYSE S. de; SOUZA, SUEDEN O. de; RAPOSO, BRUNO L.; SANTANA, GLEYCIANE S. de; SARMENTO-NETO, JOSE F.; SANTOS, BEATE S.; NEVES, REJANE P.; RIBEIRO, MARTHA S.; REBOUCAS, JULIO S.; CABRAL FILHO, PAULO E.; FONTES, ADRIANA
    Candida glabrata, a high priority fungal pathogen, presents alarming incidence and resistance potential to available antifungals. In this scenario, photodynamic inactivation (PDI) introduces a promising antifungal approach, especially in resistant cases. PDI occurs when a photosensitizer (PS) is excited by an appropriate light source, leading to production of reactive oxygen species (ROS). Zinc porphyrins (ZnPs) are attractive PSs for their efficient ROS generation and cellular uptake. The plasmonic effect of silver nanoparticles (AgNPs) can be explored to potentiate the action of PSs, such as ZnPs, in PDI. Therefore, this work aimed to study the association of AgNPs with ZnTE-2-PyP4+ (ZnP-ethyl) in PDI against C. glabrata. AgNPs were synthesized and characterized by absorption spectroscopy and electron microscopy. The association AgNP:ZnP-ethyl was evaluated by zeta potential (ζ) analyses as well as absorption and emission spectroscopies. The systems containing AgNP:ZnP-ethyl (at different proportions; v/v) and different ZnP-ethyl concentrations (0.5 – 1.5 µM) were prepared and incubated with a resistant isolate of C. glabrata, followed by illumination with a blue LED. The PDI effect was assessed by quantifying colony forming units. AgNPs presented spherical morphology and extinction maximum at ~410 nm, overlapping with the ZnP-ethyl absorption spectra. Variations in ζ confirmed the association between AgNPs and ZnP-ethyl, corroborated by subtle changes in the ZnP spectroscopic profile. The AgNP:ZnP-ethyl systems promoted complete fungal eradication at 0.6 µM, while at 0.5 µM they promoted yeast reduction of ca. 5.7 log10 (1:4; v/v) and 6.8 log10 (4:1; v/v). Illuminated AgNPs had no antimicrobial effect alone, and individual ZnP-ethyl (1.5 µM) promoted a 2 log10 reduction. These results show the benefit of AgNPs:ZnP-ethyl association to boost PDI efficiency against resistant C. glabrata isolates. This is a promising antifungal approach to treat resistant candidiasis.
  • Resumo IPEN-doc 30105
    Use of PDT therapy for symptom reduction in patients who are or have had COVID-19
    2023 - PINHEIRO, R.S.; ANA, P.A.; RIBEIRO, M.S.; TANAKA, H.
  • Artigo IPEN-doc 30044
    Avaliação eletroquímica da liga Ti6Al4V produzida por manufatura aditiva
    2023 - PIERETTI, E.F.; CORREA, O.V.; SILVA, L.C.E. da; PILLIS, M.F.; RIBEIRO, M.S.; ROSSI, W. de; NEVES, M.D.M.
    O aprimoramento de propriedades de superfície é uma exigência para osbiomateriais. O objetivo deste trabalho foi investigar a resistência à corrosãode amostras da liga Ti6Al4V produzidas por manufatura aditiva, em solução deRinger, que simula os fluidos corpóreos. As amostras foram produzidas alterando-se alguns parâmetros do processo. A resistência à corrosão foi avaliadautilizando-se métodos eletroquímicos como: monitoramento de potencial decircuito aberto, medições de espectroscopia de impedância eletroquímica epolarização cíclica e análises de superfície por MEV. Algumas amostras forammais susceptíveis à corrosão, pois a alternância de parâmetros do processo defabricação produz superfícies distintas com acabamento superficialdiversificado, resultando em comportamentos diversos.
  • Livro IPEN-doc 30000
  • Resumo IPEN-doc 29999
    Terapia fotodinâmica associada à quimioterapia em células de câncer de mama triplo-negativo
    2022 - SILVA, CAMILA R.; RIBEIRO, MARTHA S.
    INTRODUÇÃO: O câncer de mama triplo-negativo (TNBC) representa 20% das incidências de câncer de mama com uma mortalidade estimada em 40%. As principais características desse tipo de câncer seriam o seu alto poder de metástase e resistência aos tratamentos convencionais como a quimioterapia. Diante disso, há uma crescente busca por terapias adjuvantes para auxiliar o tratamento do TNBC. Nesse contexto, a terapia fotodinâmica (TFD) têm se mostrado promissora no tratamento de diversos tipos de câncer e recentemente a literatura descreve o seu uso em associação com os tratamentos convencionais para auxiliar no combate ao câncer. OBJETIVO: Associar a TFD com quimioterapia no tratamento de células TNBC. METODOLOGIA: Células TNBC, células de câncer de mama responsivas à quimioterapia (MCF-7) e células não tumorais de mama (MCF-10A) foram cultivadas, semeadas (2 x 104 células) e após a TFD usando a porfirina TMPyP (30 μM) e luz vermelha (λ= 660 ± 11 nm) com as fluências de 20 J/cm² e 50 J/cm² receberam doxorrubicina (DOX) na concentração de 5 μg/mL. Após 24-h da quimioterapia, a atividade mitocondrial foi avaliada para todos os grupos experimentais (n=6). RESULTADOS: As células MCF-7 e MCF-10A usadas como controle apresentaram redução significativa na atividade mitocondrial quando expostas à DOX. Por outro lado, as células TNBC não apresentaram redução estatisticamente significativa quando submetidas ao mesmo protocolo. No entanto, na associação da TFD+DOX, as células TNBC apresentaram redução significativa na atividade mitocondrial, independente da fluência usada. CONCLUSÃO: A associação da TFD com doxorrubicina apresentou menor atividade mitocondrial para as células TNBC.