Breast cancer estrogen and progesterone receptors evaluation using FTlR spectroscopy imaging
Carregando...
Data
Data de publicação
2020
Orientador
Título da Revista
ISSN da Revista
Título do Volume
É parte de
É parte de
É parte de
É parte de
IUPAB CONGRESS; ANNUAL SBBF MEETING; ANNUAL SBBQ MEETING
Exportar
Resumo
INTRODUCTION. Breast cancer is the second leading cause of cancer death in
woman worldwide with an incidence of 2.09 million and 627 thousand deaths in 2018.
Histopathology is the gold standard method for cancer diagnosis and identification of
therapeutic targets, however it still presents interpretation difficulties, especially when
comparing different cancer subtypes. OBJETICVE: The aim of this study was to
evaluate Fourier transform infrared (FTIR) spectroscopy in the diagnose and
differentiation of molecular differences between two different breast cancer subtypes:
positive and negative for estrogen (ER) and progesterone (PR) receptors.
METHODS: Two human breast cell lines, BT474 (ER and PR positive) and SKBR3
(ER and PR negative), were inoculated in Balb/c nude mice. Tumors were collected
when reached 0.5 cm3, processed by formalin fixation and paraffin embedding. 5μm
thick tissue cuts were fixed in low-e slides (MirrIR, Kevley Technologies). Spectral
images were performed in a micro-FTIR (Cary 660, Agilent Technologies) with
32 x 32 FPA of 5.5 μm pixel size. Scattering correction (RMieS-EMSC) was
performed using MATLAB and remaining processing using Python. Groups
differentiation were evaluated by PCA from 1350 to 1000 cm-1 second derivatives.
RESULTS AND DISCUSSION: Groups were split in two clusters, separated by PC-1
with a 99 % accuracy in both groups and 45 % of explained variance. The
absorptions in the selected region for the PCA were mainly related to DNA, RNA and
protein content. The main contribution was presented by the 1238 cm-1 peak, which
was correlated with nucleic acids symmetrical stretching. Hyperspectral image built
from this peak presented a spatial correlation with the microscope white light
imaging, indicating that possible region for histopathological correlation might be
present. CONCLUSAO: Our pilot study shows that FTIR spectroscopy imaging can
distinguish ER/PR positive from negative breast cancer subtypes.
Como referenciar
DEL-VALLE, M.; SANTOS, M.O.; SANTOS, S.N. dos; BERNARDES, E.S.; ZEZELL, D.M. Breast cancer estrogen and progesterone receptors evaluation using FTlR spectroscopy imaging: a pilot study. In: IUPAB CONGRESS; ANNUAL SBBF MEETING; ANNUAL SBBQ MEETING, October 26-30, 2020, Foz do Iguaçu, PR. Abstract... Disponível em: http://repositorio.ipen.br/handle/123456789/32354. Acesso em: 20 Mar 2026.
Esta referência é gerada automaticamente de acordo com as normas do estilo IPEN/SP (ABNT NBR 6023) e recomenda-se uma verificação final e ajustes caso necessário.