Genetic diversity of Pneumocystis jirovecii from a cluster of cases of pneumonia in renal transplant patients: Cross-sectional study
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Pneumocystis jirovecii can cause severe potentially life-threatening
pneumonia (PCP)
in kidney transplant patients. Prophylaxis of patients against PCP in this setting is
usually performed during 6 months after transplantation. The aim of this study is to
describe the molecular epidemiology of a cluster of PCP in renal transplant recipients
in Brazil. Renal transplant patients who developed PCP between May and December
2011 had their formalin-fixed
paraffin-embedded
(FFPE) lung biopsy samples analysed.
Pneumocystis jirovecii 23S mitochondrial large subunit of ribosomal RNA (23S
mtLSU-rRNA),
26S rRNA, and dihydropteroate synthase (DHPS) genes were amplified
by polymerase chain reaction (PCR), sequenced, and analysed for genetic variation.
During the study period, 17 patients developed PCP (only four infections were
documented within the first year after transplantation) and six (35.3%) died. Thirty
FFPE samples from 11 patients, including one external control HIV-infected
patient,
had fungal DNA successfully extracted for further amplification and sequencing for
all three genes. A total of five genotypes were identified among the 10 infected patients.
Of note, four patients were infected by more than one genotype and seven
patients were infected by the same genotype. DNA extracted from FFPE samples
can be used for genotyping; this approach allowed us to demonstrate that multiple
P. jirovecii strains were responsible for this cluster, and one genotype was found infecting
seven patients. The knowledge of the causative agents of PCP may help to
develop new initiatives for control and prevention of PCP among patients undergoing
renal transplant and improve routine PCP prophylaxis.
Como referenciar
RICCI, GIANNINA; SANTOS, DANIEL W.; KOVACS, JOSEPH A.; NISHIKAKU, ANGELA S.; SANDES-FREITAS, TAINA V. de; RODRIGUES, ANDERSON M.; KUTTY, GEETHA; AFFONSO, REGINA; SILVA, HELIO T.; MEDINA-PESTANA, JOSE O.; FRANCO, MARCELLO F. de; COLOMBO, ARNALDO L. Genetic diversity of Pneumocystis jirovecii from a cluster of cases of pneumonia in renal transplant patients: Cross-sectional study. Mycoses, v. 61, n. 11, p. 845-852, 2018. DOI: 10.1111/myc.12823. Disponível em: http://repositorio.ipen.br/handle/123456789/29341. Acesso em: 30 Dec 2025.
Esta referência é gerada automaticamente de acordo com as normas do estilo IPEN/SP (ABNT NBR 6023) e recomenda-se uma verificação final e ajustes caso necessário.