Molecular pathology via infrared spectral imaging for skin cancer diagnosis
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2019
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ENCONTRO DE OUTONO DA SOCIEDADE BRASILEIRA DE FÍSICA, 42.
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Resumo
Fourier Transform Infrared (FTIR) spectroscopy is a rapid and label-free analytical technique that provide
information about the overall biochemical pro¯le of biological samples based on the vibrations of its basic
molecular components (carbohydrates, proteins, lipids and nucleic acids) that are infrared active. The potential
biomedical applications of FTIR spectroscopy as a tool for cancer diagnosis have been well demonstrated over
the last years. However, most studies have focused on evaluate the diagnostic ability of FTIR by comparing
healthy tissue to cancers on advanced stage and few studies have centered on evaluating the early stages of
cancer. Thus, the present study aims to demonstrate the ability of FTIR spectroscopy to discriminate healthy
skin from advanced skin cancer (squamous cell carcinoma) as well as from early stages of malignancy using the
information retained by spectral data. Cutaneous neoplastic lesions were chemically-induced on Swiss mice using
a well-stablished two-stage carcinogenesis protocol. Healthy tissue was collected from animals non-exposed to
chemicals and di®erent disease stages were obtained by varying the exposure time of animals to carcinogenenic
factors. Tissue sections were obtained from formalin-¯xed para±n-embedded (FFPE) and placed on calcium
°uoride substrates. FTIR hyperspectral images were acquired in transmission mode over mid-infrared spectral
region and compared using Principal Components Analysis (PCA) associated to Linear Discriminant Analysis
(PC-LDA). Satisfactory data discrimination (accuracy, sensitivity and speci¯city) was achieved by PC-LDA
and the variables responsible by classi¯cation were evaluated in order to assess the spectral changes of skin
components during the transition of healthy into diseased state. Our ¯ndings demonstrate the potential of
FTIR spectroscopy not only for skin cancer diagnosis, but also to evaluate the biochemical events triggered by
cancer without requiring laborious and time-consuming procedures or expensive labeled probes as biomarkers.
Como referenciar
LIMA, CASSIO; BYRNE, HUGH; CORREA, LUCIANA; ZEZELL, DENISE. Molecular pathology via infrared spectral imaging for skin cancer diagnosis. In: ENCONTRO DE OUTONO DA SOCIEDADE BRASILEIRA DE FÍSICA, 42., 26-31 de maio, 2019, Aracaju, SE. Resumo... São Paulo: Sociedade Brasileira de Física, 2019. Disponível em: http://repositorio.ipen.br/handle/123456789/30216. Acesso em: 20 Mar 2026.
Esta referência é gerada automaticamente de acordo com as normas do estilo IPEN/SP (ABNT NBR 6023) e recomenda-se uma verificação final e ajustes caso necessário.