Photosensitization of Aggregatibacter actinomycetemcomitans with methylene blue: A microbiological and spectroscopic study

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dc.contributor.authorYAMADA JUNIOR, AECIOpt_BR
dc.contributor.authorPRATES, RENATO A.pt_BR
dc.contributor.authorCAI, SILVANApt_BR
dc.contributor.authorRIBEIRO, MARTHA S.pt_BR
dc.coverageInternacionalpt_BR
dc.date.accessioned2014-07-15T13:42:13Zpt_BR
dc.date.accessioned2014-07-30T11:48:11Z
dc.date.available2014-07-15T13:42:13Zpt_BR
dc.date.available2014-07-30T11:48:11Z
dc.date.issued2008pt_BR
dc.description.abstractThe aim of this study was to determinate the efficiency of methylene blue (MB) to kill cultures of Aggregatibacter actinomycetemcomitans under red light and to investigate MB photobleaching by optical absorption spectroscopy. Bacteria were diluted in aqueous solution, putted in glass tubes and distributed in 5 groups: (L-MB-) control group; (L+MB-) laser alone by 5min; (L-MB+) MB alone through 5min; (3L+MB+) MB+laser 3min; (5L+MB+) MB+laser 5min. Laser parameters were P=30mW, λ=660nm, E=9J in 5min and E=5.4J in 3min. The samples were diluted and bacterial colonies were counted and converted into colony forming units (CFU). Absorption spectra of the MB-stained bacterial suspension and photosensitized bacterial suspension were obtained. Groups L-MB-, L+MB-, and L-MB+ did not show a decrease in CFU/mL. L+MB+ groups showed a significant decrease in CFU/mL but no statistically significant differences were observed between 3min and 5min. Spectroscopy showed that MB is photodegraded after irradiation and that dimer species are more notably consumed than monomeric species. These results suggest that MB is a suitable photosensitizer to reduce A. actinomycetemcomitans, and that 3min of irradiation are enough to produce a significant effect. Due to the spectral changes observed on MB solution after irradiation a type I mechanism may be involved.
dc.format.extent68460F1-68460F6pt_BR
dc.identifier.citationYAMADA JUNIOR, AECIO; PRATES, RENATO A.; CAI, SILVANA; RIBEIRO, MARTHA S. Photosensitization of Aggregatibacter actinomycetemcomitans with methylene blue: A microbiological and spectroscopic study. <b>Progress in Biomedical Optics and Imaging</b>, v. 6846, p. 68460F1-68460F6, 2008. DOI: <a href="https://dx.doi.org/10.1117/12.763648">10.1117/12.763648</a>. Disponível em: http://repositorio.ipen.br/handle/123456789/4914.
dc.identifier.doi10.1117/12.763648
dc.identifier.issn1605-7422pt_BR
dc.identifier.orcidhttps://orcid.org/0000-0002-4203-1134
dc.identifier.urihttp://repositorio.ipen.br/handle/123456789/4914pt_BR
dc.identifier.vol6846pt_BR
dc.relation.ispartofProgress in Biomedical Optics and Imagingpt_BR
dc.rightsopenAccessen
dc.subjectmethylene bluept_BR
dc.subjecttherapypt_BR
dc.subjectlaserspt_BR
dc.subjectbacteriapt_BR
dc.subjectdentistrypt_BR
dc.subjectantimicrobial agentspt_BR
dc.subjectlaser radiationpt_BR
dc.subjectphotosensitivity
dc.titlePhotosensitization of Aggregatibacter actinomycetemcomitans with methylene blue: A microbiological and spectroscopic studypt_BR
dc.typeArtigo de periódicopt_BR
dspace.entity.typePublication
ipen.autorMARTHA SIMOES RIBEIRO
ipen.autorRENATO ARAÚJO PRATES
ipen.autorAECIO MASSAYOSHI YAMADA JUNIOR
ipen.codigoautor574
ipen.codigoautor4028
ipen.codigoautor3248
ipen.contributor.ipenauthorMARTHA SIMOES RIBEIRO
ipen.contributor.ipenauthorRENATO ARAÚJO PRATES
ipen.contributor.ipenauthorAECIO MASSAYOSHI YAMADA JUNIOR
ipen.date.recebimento10-05pt_BR
ipen.identifier.fiSem F.I.pt_BR
ipen.identifier.ipendoc14951pt_BR
ipen.identifier.iwosWoSpt_BR
ipen.type.genreArtigo
relation.isAuthorOfPublication36215a53-0150-4910-91d7-9559717b62d7
relation.isAuthorOfPublicationfde20222-8ca2-4a9e-941b-516590cac130
relation.isAuthorOfPublication75bfc40b-e2a3-4593-a784-f7416cfc9837
relation.isAuthorOfPublication.latestForDiscovery75bfc40b-e2a3-4593-a784-f7416cfc9837
sigepi.autor.atividadeYAMADA JUNIOR, AECIO:3248:-1:Spt_BR
sigepi.autor.atividadePRATES, RENATO A.:4028:920:Npt_BR
sigepi.autor.atividadeRIBEIRO, MARTHA S.:574:920:Npt_BR
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