Continuous and high-level in vivo delivery of endostatin from recombinant cells encapsulated in theracyte immunoisolation devices
Carregando...
Data
Data de publicação
Orientador
Título da Revista
ISSN da Revista
Título do Volume
É parte de
É parte de
É parte de
Cell Transplantation
Exportar
Resumo
Endostatin (ES) is a potent inhibitor of angiogenesis and tumor growth. Continuous ES delivery of ES
improves the efficacy and potency of the antitumoral therapy. The TheraCyte system is a polytetrafluoroethylene (PTFE) semipermeable membrane macroencapsulation system for implantation of genetically engineered cells specially designed for the in vivo delivery of therapeutic proteins, such as ES, which circumvents the problem of limited half-life and variation in circulating levels. In order to enable neovascularization
at the tissues adjacent to the devices prior to ES secretion by the cells inside them, we designed a scheme
in which empty TheraCyte devices were preimplanted SC into immunodeficient mice. Only after healing
(17 days later) were Chinese hamster ovary cells expressing ES injected into the preimplanted devices. In
another model for device implantation, the cells expressing ES where loaded into the immunoisolation devices prior to implantation into the animals, and the TheraCyte were then immediately implanted SC into
the mice. Throughout the 2-month study, constant high ES levels of up to 3.7 µg/ml were detected in the
plasma of the mice preimplanted with the devices, while lower but also constant levels of ES (up to 2.1 µg/
ml plasma) were detected in the mice that had received devices preloaded with the ES-expressing cells.
Immunohistochemistry using anti-ES antibody showed reaction within the device and outside it, demonstrating that ES, secreted by the confined recombinant cells, permeated through the membrane and reached the
surrounding tissues.
Como referenciar
MALAVASI, N.V.; RODRIGUES, D.B.; CHAMMAS, R.; CHURA-CHAMBI, R.M.; BARBUTO, J.A.M.; BALDUINO, K.; NONOGAKI, S.; MORGANTI, L. Continuous and high-level in vivo delivery of endostatin from recombinant cells encapsulated in theracyte immunoisolation devices. Cell Transplantation, v. 19, p. 269-277, 2010. DOI: 10.3727/096368909X480927. Disponível em: http://repositorio.ipen.br/handle/123456789/8129. Acesso em: 20 Mar 2026.
Esta referência é gerada automaticamente de acordo com as normas do estilo IPEN/SP (ABNT NBR 6023) e recomenda-se uma verificação final e ajustes caso necessário.