Indentity and integraty of 'alfa' and 'beta' - subunit of human thyrotropin prepared by prolonged acetic acid treatment

Página do item simplificado
dc.contributor.authorALMEIDA, BEATRIZ E.pt_BR
dc.contributor.authorCARVALHO, CRISTIANE M.pt_BR
dc.contributor.authorDAMIANI, RENATApt_BR
dc.contributor.authorOLIVEIRA, JOAO E.pt_BR
dc.contributor.authorBARTOLINI, PAOLOpt_BR
dc.contributor.authorRIBELA, MARIA T.C.P.pt_BR
dc.coverageInternacionalpt_BR
dc.creator.eventoANNUAL MEETING OF THE BRAZILIAN BIOCHEMISTRY AND MOLECULAR BIOLOGY SOCIETY, 38.pt_BR
dc.date.accessioned2014-11-19T10:36:30Zpt_BR
dc.date.accessioned2014-11-19T14:09:11Zpt_BR
dc.date.accessioned2015-04-01T13:35:13Z
dc.date.available2014-11-19T10:36:30Zpt_BR
dc.date.available2014-11-19T14:09:11Zpt_BR
dc.date.available2015-04-01T13:35:13Z
dc.date.eventoMay 16-19, 2009pt_BR
dc.description.abstractAlpha- and beta- subunits, prepared by efficiently dissociating, during 16 hours, a recombinant thyrotropin (hTSH) preparation with 0.4 M acetic acid and isolating them by RP-HPLC, were analysed for what concerns their identity and integrity. Identity was evaluated by MALDI-TOF mass spectrometry (MALDI-TOF MS). A relative molecular mass of 14021 and of 15851 was obtained for a-hTSH and (3-hTSH respectively. These values agree with those obtained by analyzing the preparation before dissociation, a difference of -1.8% for a and +1.3% for (3 being observed. Integrity of the subunits was evaluated by their capacity of self reassembling and of restoring the in vivo bioactivity of the hormone. When a-hTSH and I3-hTSH subunits were incubated together in 0.2 M sodium phosphate buffer, pH 7.0, at 25°C and under gentle shaking, a complete reassociation occurred after 4 days, forming an heterodimer. In an in vivo mouse bioassay, the T4 levels of the animals treated with the reassociated hormone were non-significantly different (p> 0.05) from those obtained when the original preparation was administered (2.71± 0.63 pg/dL versus 2.84± 0.23 pg/dL, n=6, respectively). In conclusion, subunits prepared by prolonged acetic acid treatment maintain their original molecular mass and can perfectly restore the biological activity of the reassociated heterodimers.
dc.identifier.citationALMEIDA, BEATRIZ E.; CARVALHO, CRISTIANE M.; DAMIANI, RENATA; OLIVEIRA, JOAO E.; BARTOLINI, PAOLO; RIBELA, MARIA T.C.P. Indentity and integraty of 'alfa' and 'beta' - subunit of human thyrotropin prepared by prolonged acetic acid treatment. In: ANNUAL MEETING OF THE BRAZILIAN BIOCHEMISTRY AND MOLECULAR BIOLOGY SOCIETY, 38., May 16-19, 2009, Aguas de Lindoia, SP. <b>Abstract...</b> Disponível em: http://repositorio.ipen.br/handle/123456789/19346.
dc.identifier.urihttp://repositorio.ipen.br/handle/123456789/19346pt_BR
dc.local.eventoAguas de Lindoia, SPpt_BR
dc.rightsopenAccess
dc.subjectacetic acidpt_BR
dc.subjectbiochemistrypt_BR
dc.subjectin vivopt_BR
dc.subjectmass spectroscopypt_BR
dc.subjectmicept_BR
dc.subjectstructural chemical analysispt_BR
dc.subjecttrhpt_BR
dc.subjecttshpt_BR
dc.titleIndentity and integraty of 'alfa' and 'beta' - subunit of human thyrotropin prepared by prolonged acetic acid treatmentpt_BR
dc.typeResumo de eventos científicospt_BR
dspace.entity.typePublication
ipen.autorPAOLO BARTOLINI
ipen.autorMARIA TERESA DE CARVALHO PINTO RIBELA
ipen.autorJOAO EZEQUIEL DE OLIVEIRA
ipen.autorRENATA DAMIANI
ipen.autorCRISTIANE MOREIRA DE CARVALHO
ipen.autorBEATRIZ ELANE DE ALMEIDA
ipen.codigoautor1503
ipen.codigoautor1197
ipen.codigoautor425
ipen.codigoautor3931
ipen.codigoautor4016
ipen.codigoautor6043
ipen.contributor.ipenauthorPAOLO BARTOLINI
ipen.contributor.ipenauthorMARIA TERESA DE CARVALHO PINTO RIBELA
ipen.contributor.ipenauthorJOAO EZEQUIEL DE OLIVEIRA
ipen.contributor.ipenauthorRENATA DAMIANI
ipen.contributor.ipenauthorCRISTIANE MOREIRA DE CARVALHO
ipen.contributor.ipenauthorBEATRIZ ELANE DE ALMEIDA
ipen.date.recebimento09-10pt_BR
ipen.event.datapadronizada2009pt_BR
ipen.identifier.ipendoc14160pt_BR
ipen.notas.internasAbstractpt_BR
ipen.type.genreResumo
relation.isAuthorOfPublication7d228133-8477-43fb-941d-2cfb6a48c46c
relation.isAuthorOfPublication2c1f5cfd-da46-4b49-b209-77aadfb388f1
relation.isAuthorOfPublicatione9e6346c-9b8b-4d39-8bdd-505ea6997646
relation.isAuthorOfPublication089630dd-d625-4d2a-8e26-803c1fc2243c
relation.isAuthorOfPublicationc358cfe5-abea-4abc-9224-5cac9a4f0fd1
relation.isAuthorOfPublication30050097-a62f-4d89-b572-899cdc6f3b93
relation.isAuthorOfPublication.latestForDiscovery30050097-a62f-4d89-b572-899cdc6f3b93
sigepi.autor.atividadeALMEIDA, BEATRIZ E.:6043:810:Spt_BR
sigepi.autor.atividadeCARVALHO, CRISTIANE M.:4016:-1:Npt_BR
sigepi.autor.atividadeDAMIANI, RENATA:3931:810:Npt_BR
sigepi.autor.atividadeOLIVEIRA, JOAO E.:425:810:Npt_BR
sigepi.autor.atividadeBARTOLINI, PAOLO:1503:810:Npt_BR
sigepi.autor.atividadeRIBELA, MARIA T.C.P.:1197:810:Npt_BR
Pacote Original
Agora exibindo 1 - 1 de 1
Imagem de Miniatura
Nome:
14160.pdf
Tamanho:
77.76 KB
Formato:
Adobe Portable Document Format
Baixar
Coleções
Eventos - Resumos