Effects of photodynamic inactivation mediated by Zn(II) porphyrin on promastigote and amastigote forms of Leishmania amazonensis
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Biophysical Reviews
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INTRODUCTION
Photodynamic inactivation (PDI) has been attracting attention as an innovative
technology to treat topical diseases, such as cutaneous leishmaniasis
(CL) and infections caused by multidrug-resistant microorganisms.
Zn(II) meso-tetrakis(N-n-hexylpyridinium-2-yl)porphyrin
(ZnTnHex-2-PyP 4+ ) is a lipophilic water-soluble Zn(II) porphyrin with
improved photophysical properties, high chemical stability, and cationic/
amphiphilic character that can enhance its interaction with cells.
OBJECTIVES
Thus, this study aimed to investigate the PDI effects mediated by
ZnTnHex-2-PyP 4+ on Leishmania amazonensis.
MATERIALS AND METHODS
Confocal fluorescence microscopy was explored to study the interaction
of ZnTnHex-2-PyP 4+ with promastigotes. The PDI action was analyzed
by cell membrane integrity, mitochondrial membrane potential (ΔΨm),
and cell morphology. Promastigotes were incubated with
ZnTnHex-2-PyP 4+ for 5 min at 0.62 and 1.25 μM and irradiated by a
LED (410 nm) for 1 or 3 min (2.3 and 3.4 J/cm 2 , respectively). PDI on
amastigotes and the cytotoxicity onmacrophages were also analyzed (3.4
J/cm 2 ).
DISCUSSION AND RESULTS
Fluorescence microscopy revealed that parasites efficiently uptake
ZnTnHex-2-PyP 4+ and displayed a punctate labeling pattern along with
the cytoplasm. An intenseΔΨmdepolarization was also observed, which
in association with microscopy results, suggests that ZnTnHex-2-PyP 4+
may accumulate in the mitochondrion, or other well-defined structures
close to it. Moreover, ZnTnHex-2-PyP 4+ at concentration as low as
0.62 μM led to the immediate inactivation of >95% of promastigotes,
regardless of the light dose used. Loss of the fusiform shape and plasma
membrane wrinkling were also observed. After a single treatment session
in amastigotes, PDI led to a reduction of 70% in the infection index. No
considerable toxicity was observed on mammalian cells.
CONCLUSION
Thus, PDI of Leishmania parasites showed in vitro efficiency at a
submicromolar concentration of ZnTnHex-2-PyP 4+ , with short
pre-incubation and irradiation times. The results encourage further studies
in CL pre-clinical assays and PDI of other microorganisms.
Como referenciar
SOUZA, TIAGO H. dos S.; ANDRADE, CAMILA; CABRAL, FERNANDA; SARMENTO-NETO, JOSE; REBOUCAS, JULIO; SANTOS, BEATE; RIBEIRO, MARTHA; FIGUEIREDO, REGINA; FONTES, ADRIANA. Effects of photodynamic inactivation mediated by Zn(II) porphyrin on promastigote and amastigote forms of Leishmania amazonensis. Biophysical Reviews, v. 13, n. 6, p. 1380-1381, 2021. DOI: 10.1007/s12551-021-00845-2. Disponível em: http://repositorio.ipen.br/handle/123456789/32886. Acesso em: 30 Dec 2025.
Esta referência é gerada automaticamente de acordo com as normas do estilo IPEN/SP (ABNT NBR 6023) e recomenda-se uma verificação final e ajustes caso necessário.