Iron oxide ferromagnetic nanoparticles functionalized with mPEG-CN and L-Lysine bind efficiently to cells in vitro
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2017
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PAN AMERICAN CONGRESS OF NANOTECHNOLOGY, 1st; FUNDAMENTALS AND APPLICATIONS TO SHAPE THE FUTURE
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Iron oxide nanoparticles were synthesized using an alkaline coprecipitation protocol under N2
atmosphere in order to produce cell-adherent ferromagnetic particles (CAFP). To promote
functionalization and biocompatibility, methoxypolyethylene glycol activated with cyanuric
chloride was added to nanoparticle suspensions and stirred for one hour at room temperature.
mPEG-CN is a polymer which adheres to surface of nanoparticles, improving biocompatibility. In
addition, it reacts predominantly with amine groups such as found on L-lysine, forming a
biocompatible shell. L-Lysine is well-know aminoacid whose positive (in physiologic pH) charge
will bind to cell surfaces. mPEG-CN coated particles were washed with ethanol to remove excess
and further washed three times in sterile deionized water. L-Lysine solution (1mg/mL) was added
(1:50, v/v) in five steps of five minutes each to NP and kept under sonication. Subsequently this
particle suspension was added (100μL in a 25cm2 culture flask) to a human melanoma cells (SKMEL-
37) culture and incubated for 24 hours at 37°C (5% CO2). The nanoparticles were
characterized by FTIR, x-ray diffraction and zeta potential. By optical microscopy it was possible
to verify that the nanoparticles adhered to the cell membrane and no changes in cell morphology
were observed. mPEG-CN and L-Lysine functionalization was shown to be useful to produce
CAFP. Further studies will use this protocol to perform magnetic levitation cell cultures.
Como referenciar
BONFIM, LETICIA; GONÇALVES, KARINA O.; COURROL, LILIA C.; VIEIRA, DANIEL P. Iron oxide ferromagnetic nanoparticles functionalized with mPEG-CN and L-Lysine bind efficiently to cells in vitro. In: PAN AMERICAN CONGRESS OF NANOTECHNOLOGY, 1st; FUNDAMENTALS AND APPLICATIONS TO SHAPE THE FUTURE, November 27-30, 2017, Guarujá, SP. Abstract... Disponível em: http://repositorio.ipen.br/handle/123456789/28431. Acesso em: 27 Mar 2026.
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