Protein identification from the parotoid macrogland secretion of Duttaphrynus melanostictus
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Journal of Venomous Animals and Toxins including Tropical Diseases
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Background: Bufonid parotoid macrogland secretion contains several low molecular
mass molecules, such as alkaloids and steroids. Nevertheless, its protein content is
poorly understood. Herein, we applied a sample preparation methodology that allows
the analysis of viscous matrices in order to examine its proteins.
Methods: Duttaphrynus melanostictus parotoid macrogland secretion was submitted to
ion-exchange batch sample preparation, yielding two fractions: salt-displaced fraction
and acid-displaced fraction. Each sample was then fractionated by anionic-exchange
chromatography, followed by in-solution proteomic analysis.
Results: Forty-two proteins could be identified, such as acyl-CoA-binding protein,
alcohol dehydrogenase, calmodulin, galectin and histone. Moreover, de novo analyses
yielded 153 peptides, whereas BLAST analyses corroborated some of the proteomicidentified
proteins. Furthermore, the de novo peptide analyses indicate the presence
of proteins related to apoptosis, cellular structure, catalysis and transport processes.
Conclusions: Proper sample preparation allowed the proteomic and de novo identification
of different proteins in the D. melanostictus parotoid macrogland secretion. These
results may increase the knowledge about the universe of molecules that compose
amphibian skin secretion, as well as to understand their biological/physiological role
in the granular gland.
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MARIANO, DOUGLAS O.C.; MESSIAS, MARCELA D.G.; SPENCER, PATRICK J.; PIMENTA, DANIEL C. Protein identification from the parotoid macrogland secretion of Duttaphrynus melanostictus. Journal of Venomous Animals and Toxins including Tropical Diseases, v. 25, p. 1-12, 2019. DOI: 10.1590/1678-9199-JVATITD-2019-0029. Disponível em: http://repositorio.ipen.br/handle/123456789/30470. Acesso em: 30 Dec 2025.
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