Application of egg yolk IgY on carboxylated polypyrrole films for impedimetric detection of PfHRP2 antigen
Carregando...
Data
Data de publicação
Autores IPEN
Orientador
Título da Revista
ISSN da Revista
Título do Volume
É parte de
É parte de
É parte de
Bioelectrochemistry
ODS
Exportar
Resumo
This paper described an impedimetric immunosensor for detecting Plasmodium falciparum histidine-rich protein 2 (PfHRP2). Antibodies from egg yolk (Ab-PfHRP2, IgY type) were linked covalently to the screen-printed gold electrodes (SPGE) surface modified with a thin film of Poly-pyrrole-pyrrole 3 carboxylic acid (P(Py-Py3COOH) to develop the sensing platform. The fabrication steps were followed by microscopic (scanning electron microscopy), spectroscopic (RAMAN spectroscopy and Energy-dispersive X-ray spectroscopy), and electrochemical (electrochemical impedance spectroscopy (EIS) and cyclic voltammetry) techniques. The determination of Ag-PfHRP2 was performed by EIS, and the BSA(bovine serum albumin)/Ab-PfHRP2(IgY)/P(Py-Py3COOH)/SPGE immunosensor recorded a linear response at 100–1000 ng mL−1 concentration range, with a limit of detection (LOD) of 27.47 ng mL−1. Its performance was confirmed by Enzyme-Linked Immuno-Sorbent Assay. The fabricated device uses a simple strategy of IgY immobilization, showing high sensitivity and good selectivity, and can be considered an alternative for carrying out malaria tests.
Como referenciar
GANDARILLA, ARIAMNA M.D.; GLORIA, JULIANE C.; BARCELAY, YONNY R.; SOUZA, RODRIGO F.B. de; MARIUBA, LUIS A.M.; BRITO, WALTER R. Application of egg yolk IgY on carboxylated polypyrrole films for impedimetric detection of PfHRP2 antigen. Bioelectrochemistry, v. 148, p. 1-8, 2022. DOI: 10.1016/j.bioelechem.2022.108273. Disponível em: http://repositorio.ipen.br/handle/123456789/33374. Acesso em: 30 Dec 2025.
Esta referência é gerada automaticamente de acordo com as normas do estilo IPEN/SP (ABNT NBR 6023) e recomenda-se uma verificação final e ajustes caso necessário.