Development of chitosan containing vegetable oil scaffolds by 3D printing aiming chronic epithelial wound repair
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2023
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LATIN-AMERICAN CONGRESS OF ARTIFICIAL ORGANS AND BIOMATERIALS, 12th
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Introduction and objective: Wound healing can be disrupted, slowing down the healing process and thus
resulting in chronic wounds. In the context of skin repair, chitosan is a promising natural polymer, with
antimicrobial and antioxidant properties. Such materials can form hydrogels, being suitable for 3D printing
process. Vegetable oils are in general composed of essential fatty acids, vitamins, and minerals that can
contribute to wound healing. In this work scaffolds based on chitosan incorporated with different
concentrations of vegetable oil were produced by 3D printing, aiming at the healing of chronic epithelial
wounds.
Methodology Hydrogels were produced by varying the vegetable oil percentage in the chitosan matrix. They
were used as ink for 3D printing aiming the production of scaffolds. The mechanical properties of these
structures were studied through tensile behaviour of wet and dry scaffolds. Indirect cytotoxicity, adhesion, and
proliferation assays up to 96hs were carried out using balb/c 3T3 cells to explore the biological properties of
the material.
Results and discussion The printed scaffolds presented good shape fidelity when compared to the CAD models
and displayed better results with the vegetable oil incorporation in the chitosan hydrogel. The tensile
experiments showed that the dried scaffolds have higher mechanical properties and behave as soft materials
in wet conditions. The in vitro toxicity assay results showed that the degradation products of the scaffolds were
non-toxic, and increased cell viability in a dose/dependent manner. Vegetable oil increased cell adhesion of the
chitosan scaffold, with better results at lower oil concentrations. The proliferation assay showed better results
for chitonsan containing vegetable oil in early stages. However, for 96hs, pure chitosan have better cell
proliferation.
Conclusions: Scaffolds of chitosan containing vegetable oil have potential applications as wound dressings,
acting in physical and microbiological protection and cell proliferation of the tissue. Other biological assays are
indicated to confirm the possibility of applying these biomaterials in chronic wounds.
Como referenciar
SANTOS, PAULA L. dos; LOURENCO, LARISSA; MATHOR, MONICA B.; MARCHI, JULIANA. Development of chitosan containing vegetable oil scaffolds by 3D printing aiming chronic epithelial wound repair. In: LATIN-AMERICAN CONGRESS OF ARTIFICIAL ORGANS AND BIOMATERIALS, 12th, December 12-15, 2023, Mar del Plata, Argentina. Abstract... p. 132-132. Disponível em: https://repositorio.ipen.br/handle/123456789/47941. Acesso em: 30 Apr 2026.
Esta referência é gerada automaticamente de acordo com as normas do estilo IPEN/SP (ABNT NBR 6023) e recomenda-se uma verificação final e ajustes caso necessário.