Mouse mioblast (C2C12) spheroids structured using paramagnetic iron nanoparticles as an in vitro culture system of Toxoplasma gondii tachyzoites

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dc.contributor.authorNASCIMENTO, A.C.pt_BR
dc.contributor.authorPASSOS, P.d.pt_BR
dc.contributor.authorLIMA, M.M.pt_BR
dc.contributor.authorGALISTEO JUNIOR, A.J.pt_BR
dc.contributor.authorVIEIRA, D.P.pt_BR
dc.coverageInternacionalpt_BR
dc.creator.eventoPAN-AMERICAN NANOTECHNOLOGY CONFERENCE, 2ndpt_BR
dc.date.accessioned2021-03-05T19:09:02Z
dc.date.available2021-03-05T19:09:02Z
dc.date.eventoMarch 4-7, 2020pt_BR
dc.description.abstractTridimensional cell culture techniques became essential for understanding physiological processes that are obliterated or fainted in conventional bi-dimensional cultures. These techniques are prone to produce more realistic modeling of the complex environment of living tissues, leading to much better understanding of mammalian tissue organization. This work used magnetic levitation of cell aggregates (spheroids) by adsorbing iron nanoparticles to C2C12 mouse (Mus musculus) mouse line cells (ATCC # CRL-1772), which are suspended with magnetic fields. The cells formed three-dimensional bodies that were cultivated suspended in the air-liquid interface. Magnetite (Fe3O4) nanoparticles with mean diameter of approximately 50 nm were produced by an alkaline coprecipitation methodology under reduction by microwave energy. Composition and size of crystallites were determined by DRX analysis. Adsorption on cell membranes occurred after functionalization with poly-L-lysine. Work concentrations of nanoparticles did no induce cytotoxicity in C2C12 monolayer cultures. Transmission electron microscopy of spheroid sections showed some findings morphologically compatible to the shape of reproductive intracellular vacuoli of T.gondii after cell invasion, demonstrating an interaction of cells with parasites in three-dimensional models.pt_BR
dc.event.siglaPANNANOpt_BR
dc.identifier.citationNASCIMENTO, A.C.; PASSOS, P.d.; LIMA, M.M.; GALISTEO JUNIOR, A.J.; VIEIRA, D.P. Mouse mioblast (C2C12) spheroids structured using paramagnetic iron nanoparticles as an in vitro culture system of Toxoplasma gondii tachyzoites. In: PAN-AMERICAN NANOTECHNOLOGY CONFERENCE, 2nd, March 4-7, 2020, Águas de Lindoia, SP. <b>Abstract...</b> Disponível em: http://repositorio.ipen.br/handle/123456789/31882.
dc.identifier.orcid0000-0002-0007-534Xpt_BR
dc.identifier.orcidhttps://orcid.org/0000-0002-0007-534X
dc.identifier.urihttp://repositorio.ipen.br/handle/123456789/31882
dc.local.eventoÁguas de Lindoia, SPpt_BR
dc.rightsopenAccesspt_BR
dc.titleMouse mioblast (C2C12) spheroids structured using paramagnetic iron nanoparticles as an in vitro culture system of Toxoplasma gondii tachyzoitespt_BR
dc.typeResumo de eventos científicospt_BR
dspace.entity.typePublication
ipen.autorPRISCILA DE QUEIROZ SOUZA PASSOS
ipen.autorDANIEL PEREZ VIEIRA
ipen.autorMAYELLE MARIA PAZ LIMA
ipen.autorANA CRISTINA GOMES NASCIMENTO
ipen.codigoautor14722
ipen.codigoautor3158
ipen.codigoautor14968
ipen.codigoautor14753
ipen.contributor.ipenauthorPRISCILA DE QUEIROZ SOUZA PASSOS
ipen.contributor.ipenauthorDANIEL PEREZ VIEIRA
ipen.contributor.ipenauthorMAYELLE MARIA PAZ LIMA
ipen.contributor.ipenauthorANA CRISTINA GOMES NASCIMENTO
ipen.date.recebimento21-03
ipen.event.datapadronizada2020pt_BR
ipen.identifier.ipendoc27653pt_BR
ipen.notas.internasAbstractpt_BR
ipen.type.genreResumo
relation.isAuthorOfPublicationbe35124f-007e-45eb-ba9d-2eba9d9b176d
relation.isAuthorOfPublicationc2352608-be9c-4a73-be8c-571f10bb53d2
relation.isAuthorOfPublication83bccc9e-2167-4216-a6c2-b27567054fdc
relation.isAuthorOfPublication12fd2681-92ad-4683-94d0-243825b4a97a
relation.isAuthorOfPublication.latestForDiscovery12fd2681-92ad-4683-94d0-243825b4a97a
sigepi.autor.atividadeVIEIRA, D.P.:3158:810:Npt_BR
sigepi.autor.atividadeLIMA, M.M.:14968:810:Npt_BR
sigepi.autor.atividadePASSOS, P.d.:14722:810:Npt_BR
sigepi.autor.atividadeNASCIMENTO, A.C.:14753:810:Spt_BR
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