High-level expression of mouse growth hormone (mGH) by primary human keratinocytes using a retroviral vector

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dc.contributor.authorROSAURO, C.W.pt_BR
dc.contributor.authorPERONI, C.N.pt_BR
dc.contributor.authorPOIATO, E.E.pt_BR
dc.contributor.authorSOUSA, J.M.pt_BR
dc.contributor.authorBARTOLINI, P.pt_BR
dc.coverageNacionalpt_BR
dc.creator.eventoREUNIAO ANUAL DA SOCIEDADE BRASILEIRA DE BIOQUIMICA E BIOLOGIA MOLECULAR, 31.pt_BR
dc.date.accessioned2014-11-19T11:32:03Zpt_BR
dc.date.accessioned2014-11-19T14:07:54Zpt_BR
dc.date.accessioned2015-04-01T12:45:23Z
dc.date.available2014-11-19T11:32:03Zpt_BR
dc.date.available2014-11-19T14:07:54Zpt_BR
dc.date.available2015-04-01T12:45:23Z
dc.date.evento18-21 maio, 2002pt_BR
dc.description.abstractThe skin is considered a potential target for the development of gene therapy protocols for cutaneous or systemic diseases. The high proliferative capacity of cultured keratinocytes makes these epidermal colls idoal candidates for genetie manipulation. We have utilized primary human keratinocytes transduced with an efficient retroviral vector (LXSN) encoding a mouse growth hormone gene (mGH). The main goals are the conetruction of an homologous ox vivo transfer protocol and the comparison with a similar however heterologous system based on the human growth hormone (NGH) gene. After transfection of the vector LmGHSN into GP + E-86 ecotropic packaging cell lins, tho exprassion of mGH (10ng/mL) was detected in the culture medium by a specific radioimmonuassay. This supernatant was then used to infect the amphotropic packaging cell lins GP + env Am12 and 36 clones were isolated by G418 selection. Clones presenting a secretion lovel ranging from 22-50ng mGH/10(sup)6 cells.day (44 to 150ng mGH/mL) and a viral titer of 10(sup)5 - 10(sup)6 CFU/mL were chosen to infect the keratinocytes. The modified keratinocytes presented a stable in vitro secretion level up to 3ug mGH/10(sup)6cells.day (approximately 4ug mGH/mL), during serial propagation. These results are analogous to those previously obtained for the HGH model, which was already utilized for grafting into immunodeficient dwarf mice (little/scid), Halt-ifo dotorminations for hGH and mGH are now being carried out trying to verify a possible difference of plasma clearance rate in mice, in order to correlats these data with the lvels of GH socreted in vivo by the gonotically modified keratinocytes.
dc.event.siglaSBBq,31pt_BR
dc.format.extent106pt_BR
dc.identifier.citationROSAURO, C.W.; PERONI, C.N.; POIATO, E.E.; SOUSA, J.M.; BARTOLINI, P. High-level expression of mouse growth hormone (mGH) by primary human keratinocytes using a retroviral vector. In: REUNIAO ANUAL DA SOCIEDADE BRASILEIRA DE BIOQUIMICA E BIOLOGIA MOLECULAR, 31., 18-21 maio, 2002, Caxambu, MG. <b>Resumos...</b> p. 106. Disponível em: http://repositorio.ipen.br/handle/123456789/20940.
dc.identifier.orcidhttps://orcid.org/0000-0001-8194-5230
dc.identifier.urihttp://repositorio.ipen.br/handle/123456789/20940pt_BR
dc.local.eventoCaxambu, MGpt_BR
dc.publisherSao Paulo: SBBq, 2002pt_BR
dc.rightsopenAccess
dc.subjectsthpt_BR
dc.subjectmicept_BR
dc.subjectepidermispt_BR
dc.subjectanimal cellspt_BR
dc.subjectcloningpt_BR
dc.titleHigh-level expression of mouse growth hormone (mGH) by primary human keratinocytes using a retroviral vectorpt_BR
dc.typeResumo de eventos científicospt_BR
dspace.entity.typePublication
ipen.autorPAOLO BARTOLINI
ipen.autorJOSE MARIA DE SOUSA
ipen.autorCIBELE NUNES PERONI
ipen.autorCRISTIANE WANDERLEY ROSAURO
ipen.codigoautor1503
ipen.codigoautor69
ipen.codigoautor947
ipen.codigoautor2866
ipen.contributor.ipenauthorPAOLO BARTOLINI
ipen.contributor.ipenauthorJOSE MARIA DE SOUSA
ipen.contributor.ipenauthorCIBELE NUNES PERONI
ipen.contributor.ipenauthorCRISTIANE WANDERLEY ROSAURO
ipen.date.recebimento03-03pt_BR
ipen.event.datapadronizada2002pt_BR
ipen.identifier.ipendoc08538pt_BR
ipen.notas.internasResumospt_BR
ipen.type.genreResumo
relation.isAuthorOfPublication7d228133-8477-43fb-941d-2cfb6a48c46c
relation.isAuthorOfPublicationf5a86069-5228-4532-b51f-f5218f073b98
relation.isAuthorOfPublicationc30bdc49-9059-4fa3-91a9-7c92d7cd1dab
relation.isAuthorOfPublicationad0b47ea-7655-43ed-b34b-e81a8ac9d8e5
relation.isAuthorOfPublication.latestForDiscoveryad0b47ea-7655-43ed-b34b-e81a8ac9d8e5
sigepi.autor.atividadeROSAURO, C.W.:2866:-1:Spt_BR
sigepi.autor.atividadePERONI, C.N.:947:18:Npt_BR
sigepi.autor.atividadePOIATO, E.E.:-1:-1:Npt_BR
sigepi.autor.atividadeSOUSA, J.M.:69:18:Npt_BR
sigepi.autor.atividadeBARTOLINI, P.:1503:18:Npt_BR
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