Study of irradiated bothropstoxin-1 with sup(6)Co gamma rays: immune system behavior

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dc.contributor.authorCAPRONI, P.pt_BR
dc.contributor.authorBAPTISTA, J.A.pt_BR
dc.contributor.authorALMEIDA, T.L. dept_BR
dc.contributor.authorPASSOS, L.A.C.pt_BR
dc.contributor.authorNASCIMENTO, N.pt_BR
dc.coverageInternacionalpt_BR
dc.date.accessioned2014-07-15T13:41:45Zpt_BR
dc.date.accessioned2014-07-30T11:50:58Z
dc.date.available2014-07-15T13:41:45Zpt_BR
dc.date.available2014-07-30T11:50:58Z
dc.date.issued2009pt_BR
dc.description.abstractIonizing radiation has been successfully employed to modify the immunological properties of biomolecules. Very promising results were obtained when crude animal venoms, as well as isolated toxins, were treated with 60Co gamma rays, yielding toxoids with good immunogenicity. The achievement of modified antigens with lower toxicity and preserved or improved immunogenicity can be very useful. Ionizing radiation has already been proven to be a powerful tool to attenuate snake venom toxicity without affecting, and even increasing, their immunogenic properties. However, little is known about the modifications that irradiated molecules undergo and even less about the immunological response that such antigens elicit. In the present work, we investigated the immunological behavior of bothropstoxin-1, a K49 phospholipase, before and after irradiation. Structural modifications of the toxin were analyzed by SDS-PAGE. Isogenic mice were immunized with either the native or the irradiated toxin. The circulating antibodies were isotyped and titrated by ELISA. According to our data, irradiation promoted structural modifications in the toxin characterized by higher molecular weight forms of proteins (aggregates and oligomers). The results also indicated that irradiated toxins were immunogenic and antibodies elicited by them were able to recognize the native toxin in ELISA. These findings suggest that irradiation of toxic proteins can promote significant modifications in their structures; however they still retain many of the original antigenic and immunological properties of native proteins. Also, our data indicate that irradiated proteins induce higher titers of IgG2a and IgG2b, suggesting that Th1 cells are predominantly involved in the immune response.
dc.format.extent216-225pt_BR
dc.identifier.citationCAPRONI, P.; BAPTISTA, J.A.; ALMEIDA, T.L. de; PASSOS, L.A.C.; NASCIMENTO, N. Study of irradiated bothropstoxin-1 with sup(6)Co gamma rays: immune system behavior. <b>Journal of Venomous Animals and Toxins Including Tropical Diseases</b>, v. 15, n. 2, p. 216-225, 2009. DOI: <a href="https://dx.doi.org/10.1590/S1678-91992009000200005">10.1590/S1678-91992009000200005</a>. Disponível em: http://repositorio.ipen.br/handle/123456789/4872.
dc.identifier.doi10.1590/S1678-91992009000200005
dc.identifier.fasciculo2pt_BR
dc.identifier.issn1678-9199pt_BR
dc.identifier.urihttp://repositorio.ipen.br/handle/123456789/4872pt_BR
dc.identifier.vol15pt_BR
dc.relation.ispartofJournal of Venomous Animals and Toxins Including Tropical Diseasespt_BR
dc.rightsopenAccessen
dc.subjectbiological radiation effectspt_BR
dc.subjectcobalt 60pt_BR
dc.subjectgamma radiationpt_BR
dc.subjectimmunologypt_BR
dc.subjectpathological changespt_BR
dc.subjectsnakespt_BR
dc.subjecttoxinspt_BR
dc.titleStudy of irradiated bothropstoxin-1 with sup(6)Co gamma rays: immune system behaviorpt_BR
dc.typeArtigo de periódicopt_BR
dspace.entity.typePublication
ipen.autorNANCI DO NASCIMENTO
ipen.autorTIAGO LUIZ DE ALMEIDA
ipen.autorJANAINA BAPTISTA ALVES
ipen.autorPRISCILA CAPRONI
ipen.codigoautor411
ipen.codigoautor3919
ipen.codigoautor3061
ipen.codigoautor3462
ipen.contributor.ipenauthorNANCI DO NASCIMENTO
ipen.contributor.ipenauthorTIAGO LUIZ DE ALMEIDA
ipen.contributor.ipenauthorJANAINA BAPTISTA ALVES
ipen.contributor.ipenauthorPRISCILA CAPRONI
ipen.date.recebimento09-10pt_BR
ipen.identifier.fi0.479pt_BR
ipen.identifier.ipendoc14083pt_BR
ipen.identifier.iwosWoSpt_BR
ipen.range.fi0.001 - 1.499
ipen.type.genreArtigo
relation.isAuthorOfPublication53eed426-0bd7-4230-8b8c-f48795fad345
relation.isAuthorOfPublication456d319a-a1bc-450f-88aa-6b16fcf88ae7
relation.isAuthorOfPublication68a92216-61bb-4e06-b42e-bfebcd5380d9
relation.isAuthorOfPublication7d5a8a7b-9d65-4586-a6f7-9f7a3644fdd9
relation.isAuthorOfPublication.latestForDiscovery7d5a8a7b-9d65-4586-a6f7-9f7a3644fdd9
sigepi.autor.atividadeCAPRONI, P.:3462:820:Spt_BR
sigepi.autor.atividadeBAPTISTA, J.A.:3061:820:Npt_BR
sigepi.autor.atividadeALMEIDA, T.L. DE:3919:230:Npt_BR
sigepi.autor.atividadeNASCIMENTO, N.:411:820:Npt_BR
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