Determination of radioresistance of contaminant bacteria in nile tilapia skin used as a dressing
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2023
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LATIN-AMERICAN CONGRESS OF ARTIFICIAL ORGANS AND BIOMATERIALS, 12th
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Introduction and objective: The use of Nile Tilapia (Oreochromis niloticus) skin in regenerative medicine, in
the form of biological dressing for burns, is satisfactory because this biomaterial presents good adherence
to wounds and characteristics suitable for the healing process, such as the high concentration of collagen
type I. The treatment with the skin provides protection and decreases the number of dressing changes,
reducing the patient's pain. Before use, Nile Tilapia skins need to be decontaminated and sterilized ensuring
safety, since they can be contaminated by bacteria during handling. Each bacterium has a radioresistance
value, which inactivates 90% of the population of that species.
Methodology: Some human contaminations at the time of handling this material: Enterococcus faecalis,
Pseudomonas aeruginosa and Pseudomonas putida. In this work we irradiated the isolated bacterial
populations from the Nile Tilapia skin with increasing doses of ionizing radiation in culture medium. Serial
dilutions were performed from the total population, after gamma-ray irradiation, to enable the identification
of the remaining bacterial concentration for each dose of irradiation tested.
Results and discussion: In the literature it is possible to find radioresistance to these bacteria contained in
other substrates. However, it is necessary to standardize the methodology to determine the radioresistance
of microbial species in the skin of Nile Tilapia, to ensure patient safety during the use of the dressing. For
Enterococcus faecalis we obtained a value of 0.7 kGy, very similar to that described in the literature. For
Pseudomonas aeruginosa the value found was 0.5 kGy and for Pseudomonas putida it was 0.2 kGy. Thus, the
standardization of the methodology is in accordance with normality, also allowing the determination of
radioresistance for some bacteria not described in the literature.
Conclusions: It was possible to estimate the values of the doses necessary for the reduction of 90% of the
bacterial population. The radiation doses were standardized to sterilize with a safety level of 10-6, but
without reaching values harmful to the histological and molecular structure of the biomaterial (above 25
kGy), so that the biological dressing is used in patients safely and doesn’t lose its physicochemical
characteristics.
Como referenciar
BARNA, FERNANDA S.; BANDEIRA, TEREZA J.P.G.; PAIER, CARLOS R.K.; RODRIGUES, FELIPE A.R.; LIMA JUNIOR, EDMAR M.; MARAES FILHO, MANUEL O. de; MATHOR, MONICA B. Determination of radioresistance of contaminant bacteria in nile tilapia skin used as a dressing. In: LATIN-AMERICAN CONGRESS OF ARTIFICIAL ORGANS AND BIOMATERIALS, 12th, December 12-15, 2023, Mar del Plata, Argentina. Abstract... p. 205-205. Disponível em: https://repositorio.ipen.br/handle/123456789/47937. Acesso em: 13 Mar 2025.
Esta referência é gerada automaticamente de acordo com as normas do estilo IPEN/SP (ABNT NBR 6023) e recomenda-se uma verificação final e ajustes caso necessário.