Synthesis and characterization of 5-ALA gold nanoparticles
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2017
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INTERNATIONAL CONFERENCE ON LUMINESCENCE, 18th
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Atherosclerosis is a chronic inflammatory disease and the main cause of human death worldwide. Studies have proposed that macrophages play a significant role in the development, progression, and destabilization of atherosclerotic plaques[1]. Thus, reduction of macrophages from plaque represents a new strategy for the treatment of atherosclerosis. Photodynamic and Sonodynamic therapies (PDT and SDT, respectively) are emerging as new atherosclerosis treatments [1, 2]. The subsequent generation of free radicals by activated photo and sonosensitizers can lead to apoptotic cell death. The use of gold nanoparticles (AuNPs) as the vehicle for a sensitizer delivery improves reactive oxygen species formation [3]. Previous works demonstrated that aminolevulinic acid (5-ALA) mediated PDT could reduce macrophage content and inhibit plaque progression, indicating a promising approach to treat inflamed atherosclerotic plaque[4]. In this study ALA:AuNps functionalized with polyethylene glycol (PEG) were synthesized mixing 5-ALA with tetrachloroauric(III) acid in milliQ water solution followed by photo reduction with 300 Watts Xenon lamp. The synthesized nanoparticles were characterized by UV/Vis optical absorption, and electron microscopy. Singlet oxygen generation efficiency was measured by trapping with 1,3-diphenylisobenzofuran for irradiations with 590 nm LED (~100 mW) and pulse ultrasound irradiation (1 and 3W/cm2 with 1.0 and 2.0 MHz ). The potential use of ALA:AuNps as a sensitizer for photo and sonodynamic therapies were investigated on THP-1 macrophage. Cytotoxicity test were also described. The findings suggested that ALA:AuNps under LED illumination and ultrasound had photo and sonodynamic effect on THP-1 macrophages via generation of intracellular singlet oxygen, indicating that ALA:AuNps could be used as a novel photo and sonosensitizer for atherosclerosis.
Como referenciar
OLIVEIRA, K.G. de; VIEIRA, D.P.; COURROL, L.C. Synthesis and characterization of 5-ALA gold nanoparticles: photo and sonosensitizer agent for atherosclerosis. In: INTERNATIONAL CONFERENCE ON LUMINESCENCE, 18th, August 27 - September 01, 2017, João Pessoa, PB. Abstract... São Paulo, SP: Sociedade Brasileira de Química, 2017. p. 304-304. Disponível em: http://repositorio.ipen.br/handle/123456789/28606. Acesso em: 29 Apr 2026.
Esta referência é gerada automaticamente de acordo com as normas do estilo IPEN/SP (ABNT NBR 6023) e recomenda-se uma verificação final e ajustes caso necessário.