Bovine serum albumin conjugated gold-198 nanoparticles as model to evaluate the damage caused by ionizing radiation to biomolecules

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dc.contributor.authorSANTOS, JONNATAN J.
dc.contributor.authorLEAL, JESSICA
dc.contributor.authorDIAS, LUIS A.P.
dc.contributor.authorTOMA, SERGIO H.
dc.contributor.authorCORIO, PAOLA
dc.contributor.authorGENEZINI, FREDERICO A.
dc.contributor.authorKATTI, KATTESH V.
dc.contributor.authorARAKI, KOITI
dc.contributor.authorLUGAO, ADEMAR B.
dc.coverageInternacionalpt_BR
dc.date.accessioned2018-09-03T11:25:09Z
dc.date.available2018-09-03T11:25:09Z
dc.date.issued2018pt_BR
dc.description.abstractGold nanoparticles (AuNPs) have several applications including in medicine. Considering cancer as one of the most common diseases for men and women, new treatments and more specific and effective drugs, which cause less side effects, have been actively pursued. Among them, gold-198 can be engineered as theranostic agents, working as contrast (exploiting gamma emission) and treatment agents (beta emission). Accordingly, a new procedure for the production of 14 nm diameter radioactive citrate protected gold-198 nanoparticles, that were then conjugated with bovine serum albumin utilizing 3-mercaptopropionic acid directly bound to AuNPs surface as anchoring groups, generating fully dispersible nanoparticles in aqueous media, are described. The effect of gamma and beta radiation on grafted BSA was evaluated by direct irradiation of the corresponding cold material and comparing with the damage caused on BSA grafted gold-198 nanoparticles prepared from a neutron activated gold foil. The investigation by fluorescence and Raman spectroscopy indicated that the damage to BSA chromophore groups is proportional to the dose (from 0.1 to 1 kGy) and that chromophores groups close to the particle surface are more prone to damage. Gold-198 nanoparticles conjugated with bovine serum albumin showed that process is much more localized next to nanoparticles surface since each gold core acts as a punctual radiation source. In short, AuNPs can enhance the damage caused by irradiation of cold nanoparticles and AuNPs@MPA-BSA is a suitable model to probe the effect of gamma and beta emitter on biomolecules. Furthermore, the strategy of diluting the gold-198 with cold gold atoms was shown to be suitable to control the activity of 198AuNPs aiming medical applications, since the damage to BSA was found to be proportional to the relative concentration of gold-198.pt_BR
dc.identifier.citationSANTOS, JONNATAN J.; LEAL, JESSICA; DIAS, LUIS A.P.; TOMA, SERGIO H.; CORIO, PAOLA; GENEZINI, FREDERICO A.; KATTI, KATTESH V.; ARAKI, KOITI; LUGAO, ADEMAR B. Bovine serum albumin conjugated gold-198 nanoparticles as model to evaluate the damage caused by ionizing radiation to biomolecules. <b>ACS Applied Nano Materials</b>, 2018. DOI: <a href="https://dx.doi.org/10.1021/acsanm.8b01174">10.1021/acsanm.8b01174</a>. Disponível em: http://repositorio.ipen.br/handle/123456789/29074.
dc.identifier.doi10.1021/acsanm.8b01174pt_BR
dc.identifier.issn2574-0970pt_BR
dc.identifier.orcidhttps://orcid.org/0000-0002-1737-3191
dc.identifier.orcidhttps://orcid.org/0000-0002-6318-6805
dc.identifier.percentilfiSem Percentilen
dc.identifier.percentilfiCiteScoreSem Percentil CiteScore
dc.identifier.urihttp://repositorio.ipen.br/handle/123456789/29074
dc.relation.ispartofACS Applied Nano Materialspt_BR
dc.rightsclosedAccesspt_BR
dc.subjectgold 198
dc.subjectcattle
dc.subjectblood serum
dc.subjectalbumins
dc.subjectnanoparticles
dc.subjectionizing radiations
dc.subjecttherapy
dc.subjectgamma spectroscopy
dc.subjectneoplasms
dc.titleBovine serum albumin conjugated gold-198 nanoparticles as model to evaluate the damage caused by ionizing radiation to biomoleculespt_BR
dc.typeArtigo de periódicopt_BR
dspace.entity.typePublication
ipen.autorJESSICA LEAL
ipen.autorADEMAR BENEVOLO LUGAO
ipen.autorFREDERICO ANTONIO GENEZINI
ipen.autorLUIS ALBERTO PEREIRA DIAS
ipen.autorJONNATAN JULIVAL DOS SANTOS
ipen.codigoautor11679
ipen.codigoautor339
ipen.codigoautor2045
ipen.codigoautor796
ipen.codigoautor14395
ipen.contributor.ipenauthorJESSICA LEAL
ipen.contributor.ipenauthorADEMAR BENEVOLO LUGAO
ipen.contributor.ipenauthorFREDERICO ANTONIO GENEZINI
ipen.contributor.ipenauthorLUIS ALBERTO PEREIRA DIAS
ipen.contributor.ipenauthorJONNATAN JULIVAL DOS SANTOS
ipen.date.recebimento18-09pt_BR
ipen.identifier.fiSem F.I.pt_BR
ipen.identifier.fiCiteScoreSem CiteScore
ipen.identifier.ipendoc24857pt_BR
ipen.identifier.ods3
ipen.type.genreArtigo
relation.isAuthorOfPublication4718f72f-9574-4e63-8272-371bfddc8de6
relation.isAuthorOfPublication99ac24c5-2ae1-465a-a6f2-40b4d9af6af7
relation.isAuthorOfPublication0c41d307-45a9-47c4-8281-5aa9ed46e6a4
relation.isAuthorOfPublication50b3adab-a651-4225-a05c-ec1b1e37e921
relation.isAuthorOfPublicationc7b4987e-3364-4802-b86a-be5795156c8d
relation.isAuthorOfPublication.latestForDiscoveryc7b4987e-3364-4802-b86a-be5795156c8d
sigepi.autor.atividadeSANTOS, JONNATAN J.:14395:740:Spt_BR
sigepi.autor.atividadeLEAL, JESSICA:11679:740:Npt_BR
sigepi.autor.atividadeDIAS, LUIS A.P.:796:110:Npt_BR
sigepi.autor.atividadeGENEZINI, FREDERICO A.:2045:310:Npt_BR
sigepi.autor.atividadeLUGAO, ADEMAR B.:339:740:Npt_BR
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