Cell cycle arrest, extracellular matrix changes and intrinsic apoptosis in human melanoma cells are induced by boron neutron capture therapy
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2013
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Toxicology in Vitro
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Boron Neutron Capture Therapy (BNCT) involves the selective accumulation of boron carriers in tumor
tissue followed by irradiation with a thermal or epithermal neutron beam. This therapy is therefore a cellular irradiation suited to treat tumors that have infiltrated into healthy tissues. BNCT has been used clinically to treat patients with cutaneous melanomas which have a high mortality. Human normal
melanocytes and melanoma cells were treated with BNCT at different boronophenylalanine concentrations for signaling pathways analysis. BNCT induced few morphological alterations in normal melanocytes, with a negligible increase in free radical production. Melanoma cells treated with BNCT showed
significant extracellular matrix (ECM) changes and a significant cyclin D1 decrease, suggesting cell death
by necrosis and apoptosis and cell cycle arrest, respectively. BNCT also induced a significant increase in
cleaved caspase-3 and a decrease in the mitochondrial electrical potential with selectivity for melanoma
cells. Normal melanocytes had no significant differences due to BNCT treatment, confirming the data
from the literature regarding the selectivity of BNCT. The results from this study suggest that some signaling pathways are involved in human melanoma treatment by BNCT, such as cell cycle arrest, ECM
changes and intrinsic apoptosis.
Como referenciar
FAIAO FLORES, FERNANDA; COELHO, PAULO R.P.; ARRUDA NETO, JOAO D.T.; MARIA ENGLER, SILVYA S.; MARIA, DURVANEI A. Cell cycle arrest, extracellular matrix changes and intrinsic apoptosis in human melanoma cells are induced by boron neutron capture therapy. Toxicology in Vitro, v. 27, n. 4, p. 1196-1204, 2013. DOI: 10.1016/j.tiv.2013.02.006. Disponível em: http://repositorio.ipen.br/handle/123456789/8513. Acesso em: 27 Mar 2025.
Esta referência é gerada automaticamente de acordo com as normas do estilo IPEN/SP (ABNT NBR 6023) e recomenda-se uma verificação final e ajustes caso necessário.