Light parameters influence cell viability in antifungal photodynamic therapy in a fluence and rate fluence-dependent manner

Página do item simplificado
dc.contributor.authorPRATES, RENATO A.pt_BR
dc.contributor.authorSILVA, ERIQUES G. dapt_BR
dc.contributor.authorYAMADA JUNIOR, AECIO M.pt_BR
dc.contributor.authorSUZUKI, LUIS C.pt_BR
dc.contributor.authorPAULA, CLAUDETE R.pt_BR
dc.contributor.authorRIBEIRO, MARTHA S.pt_BR
dc.coverageInternacionalpt_BR
dc.date.accessioned2014-07-15T13:41:43Zpt_BR
dc.date.accessioned2014-07-30T11:50:32Z
dc.date.available2014-07-15T13:41:43Zpt_BR
dc.date.available2014-07-30T11:50:32Z
dc.date.issued2009pt_BR
dc.description.abstractThe aim of this study was to investigate the influence of light parameters on yeast cells. It has been proposed for many years that photodynamic therapy (PDT) can inactivate microbial cells. A number of photosensitizer and light sources were reported in different light parameters and in a range of dye concentrations. However, much more knowledge concerning the importance of fluence, fluence rate and exposure time are required for a better understanding of the photodynamic efficiency. Suspensions (106 CFU/mL) of Candida albicans, Candida krusei, and Cryptococcus neoformans var. grubii were used. Two fluence rates, 100 and 300 mW/cm2 were compared at 3, 6, and 9 min of irradiation, resulting fluences from 18 to 162 J/cm2. The light source was a laser emitting at λ = 660 nm with output power adjusted at 30 and 90 mW. As photosensitizer, one hundred-μM methylene blue was used. Temperature was monitored to verify possible heat effect and reactive oxygen species (ROS) formation was evaluated. The same fluence in different fluence rates showed dissimilar levels of inactivation on yeast cells as well as in ROS formation. In addition, the increase of the fluence rate showed an improvement on cell photoinactivation. PDT was efficient against yeast cells (6 log reduction), and no significant temperature increase was observed. Fluence per se should not be used as an isolate parameter to compare photoinactivation effects on yeast cells. The higher fluence rate was more effective than the lower one. Furthermore, an adequate duration of light exposure cannot be discarded.
dc.format.extent1038-1044pt_BR
dc.identifier.citationPRATES, RENATO A.; SILVA, ERIQUES G. da; YAMADA JUNIOR, AECIO M.; SUZUKI, LUIS C.; PAULA, CLAUDETE R.; RIBEIRO, MARTHA S. Light parameters influence cell viability in antifungal photodynamic therapy in a fluence and rate fluence-dependent manner. <b>Laser Physics</b>, v. 19, n. 5, p. 1038-1044, 2009. DOI: <a href="https://dx.doi.org/10.1134/S1054660X09050284">10.1134/S1054660X09050284</a>. Disponível em: http://repositorio.ipen.br/handle/123456789/4869.
dc.identifier.doi10.1134/S1054660X09050284
dc.identifier.fasciculo5pt_BR
dc.identifier.issn1054-660Xpt_BR
dc.identifier.orcidhttps://orcid.org/0000-0002-4203-1134
dc.identifier.urihttp://repositorio.ipen.br/handle/123456789/4869pt_BR
dc.identifier.vol19pt_BR
dc.relation.ispartofLaser Physicspt_BR
dc.rightsclosedAccessen
dc.subjectyeastspt_BR
dc.subjectanimal cellspt_BR
dc.subjectfungal diseasespt_BR
dc.subjectmicroorganismspt_BR
dc.subjectphotosensitivity
dc.subjecttherapy
dc.titleLight parameters influence cell viability in antifungal photodynamic therapy in a fluence and rate fluence-dependent mannerpt_BR
dc.typeArtigo de periódicopt_BR
dspace.entity.typePublication
ipen.autorMARTHA SIMOES RIBEIRO
ipen.autorLUIS CLAUDIO SUZUKI
ipen.autorAECIO MASSAYOSHI YAMADA JUNIOR
ipen.autorRENATO ARAÚJO PRATES
ipen.codigoautor574
ipen.codigoautor3953
ipen.codigoautor3248
ipen.codigoautor4028
ipen.contributor.ipenauthorMARTHA SIMOES RIBEIRO
ipen.contributor.ipenauthorLUIS CLAUDIO SUZUKI
ipen.contributor.ipenauthorAECIO MASSAYOSHI YAMADA JUNIOR
ipen.contributor.ipenauthorRENATO ARAÚJO PRATES
ipen.date.recebimento09-10pt_BR
ipen.identifier.fi0.676pt_BR
ipen.identifier.ipendoc14087pt_BR
ipen.identifier.iwosWoSpt_BR
ipen.range.fi0.001 - 1.499
ipen.type.genreArtigo
relation.isAuthorOfPublication36215a53-0150-4910-91d7-9559717b62d7
relation.isAuthorOfPublication27acc312-4d9c-45d2-bfa7-b51e8f00f5c0
relation.isAuthorOfPublication75bfc40b-e2a3-4593-a784-f7416cfc9837
relation.isAuthorOfPublicationfde20222-8ca2-4a9e-941b-516590cac130
relation.isAuthorOfPublication.latestForDiscoveryfde20222-8ca2-4a9e-941b-516590cac130
sigepi.autor.atividadePRATES, RENATO A.:4028:920:Spt_BR
sigepi.autor.atividadeYAMADA JUNIOR, AECIO M.:3248:-1:Npt_BR
sigepi.autor.atividadeSUZUKI, LUIS C.:3953:920:Npt_BR
sigepi.autor.atividadeRIBEIRO, MARTHA S.:574:920:Npt_BR
Pacote Original
Agora exibindo 1 - 1 de 1
Nenhuma Miniatura disponível
Nome:
14087.pdf
Tamanho:
183.41 KB
Formato:
Adobe Portable Document Format
Baixar
Coleções
Periódicos