Enhancement of human prolactin synthesis by sodium butyrate addition to serum-free CHO cell culture
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Journal of Biomedicine and Biotechnology
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Sodium butyrate (NaBu) has been used as a productivity enhancer for the synthesis of recombinant proteins in Chinese hamster
ovary (CHO) cells. Thus, the influence of NaBu on the production of recombinant human prolactin (hPRL) from CHO cells was
investigated for the first time. CHO cell cultures were submitted to a treatment with different concentrations of NaBu (0.25 to
4 mM). Quantitative and qualitative analyses by reverse-phase high-performance liquid chromatography (RP-HPLC) and Western
blot or SDS-PAGE, carried out directly on CHO-conditioned medium, showed that the highest hPRL expression was obtained
with 1 mM NaBu. In vitro biological assays based on noble rat lymphoma (Nb2) and mouse pro-B lymphoma (Ba/F3-LLP) cells
were carried out on purified hPRL. Its bioactivity in the presence of NaBu was not apparently different from that of the First
International Reference Reagent of recombinant hPRL (WHO 97/714). Our results show that NaBu increased the synthesis of
recombinant hPRL in CHO cells, apparently without compromising either its structure or function.
Como referenciar
GOULART, HERBERT R.; ARTHUSO, FERNANDA dos S.; CAPONE, MARCOS V.N.; OLIVEIRA, TAIS L. de; BARTOLINI, PAOLO; SOARES, CARLOS R.J. Enhancement of human prolactin synthesis by sodium butyrate addition to serum-free CHO cell culture. Journal of Biomedicine and Biotechnology, v. 2010, p. 405872-1 - 405872-11, 2010. DOI: 10.1155/2010/405872. Disponível em: http://repositorio.ipen.br/handle/123456789/4704. Acesso em: 20 Mar 2026.
Esta referência é gerada automaticamente de acordo com as normas do estilo IPEN/SP (ABNT NBR 6023) e recomenda-se uma verificação final e ajustes caso necessário.