CXCL12 expression in hematopoitic tissues of mice exposed to sublethal dose of ionizing radiations in the presence of iNOS inhibitor
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2005
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INTERNATIONAL NUCLEAR ATLANTIC CONFERENCE; ENCONTRO NACIONAL DE APLICACOES NUCLEARES, 7th
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We study the production of CXCL12, a stem cell homing chemokine, in spleen and bone
marrow of mice exposed at LD50% of γ-radiation, w/wo a iNOS blocker,
aminoguanidine, to test if inflammatory nitric oxide is involved in necrotic processes of
stem cell death after ionizing radiation exposure. Groups of 10 male 6-week old C57Bl/6j
mice were killed at specific time points after a 8Gy dose irradiation (60Co source;
4,22kGy/h dose rate) and spleen and bone marrow samples were immersed and stored
in TriZOL® for total mRNA extraction. RT-PCR assays were performed to determine the
production of CXCL12 as compared to murine β-actin at days 2th, 5 th, 7 th, 9 th and 15 th
days after radiation in a semiquantitative way. PCR was performed after cDNA synthesis
using Oligo-dT primers and specific primers for CXCL12 and β-actin. Artificial optical
density was determined in silver-stained PAGE resolved specific amplification products
of CXCL12, using amplification of murine β-actin as standard, and measurements
obtained by the Image J freeware. CXCL12 production in spleen samples reached its
maximum at 5 th day after radiation exposure in animals not treated with
aminoguanidine, but this peak was extended to at 7 th day in treated animals. Nontreated animals presented a decrease of CXCL12 expression up to 15 th day of
experiment, and aminoguanidine treated animals showed sustained increase of
expression levels between 9 th and 15 th days. In bone marrow samples, the main
difference among the two different experimental groups was a maintenance of CXCL12
mRNA expression between 7 th and 9 th days, persisting until the end of the experiment.
Our data demonstrates that the effect of aminoguanidine appears to sustain the
CXCL12 mRNA synthesis in hematopoetic tissues of irradiated mice, providing some
evidences that the axis iNOS –NO – inflammation must be involved in stem cell death,
aside to the direct radiation effect, suggesting their use associated to the CXCL12
chemokine to enhance the post-transplantation grafting of hematopoietic tissue and/or
treatment of accidentaly exposed individuals to sublethal doses of ionizing radiation.
Como referenciar
PEREZ, D.; HERMIDA, F.P.M.; ANDRADE JUNIOR, H.F. CXCL12 expression in hematopoitic tissues of mice exposed to sublethal dose of ionizing radiations in the presence of iNOS inhibitor. In: INTERNATIONAL NUCLEAR ATLANTIC CONFERENCE; ENCONTRO NACIONAL DE APLICACOES NUCLEARES, 7th, ago. 28 - set. 2, 2005, Santos, SP. Resumos... Disponível em: http://repositorio.ipen.br/handle/123456789/19925. Acesso em: 26 Mar 2026.
Esta referência é gerada automaticamente de acordo com as normas do estilo IPEN/SP (ABNT NBR 6023) e recomenda-se uma verificação final e ajustes caso necessário.