The impact of physical-chemical parameters over the formation of papain nanoparticles crosslinked by radiation
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2017
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PAN AMERICAN CONGRESS OF NANOTECHNOLOGY, 1st
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With the advancement of research and use of nanotechnology in biomedical areas and
application in different treatments the need for stable systems with low toxicity and null adverse
effects becomes important. Within this context papain appears as an alternative biomolecule
which is a proteolytic enzyme extract by the fruit of Carica papaya Linnaeus. Its already
successfully applied in the pharmaceutical and biomedical area as a drug carrier and debriding
agent for wounds. Recently, papain was developed in nanoscale using 50 Mm phosphate buffer
(pH 7,2 ± 0,2), ethanol (20%, v/v) as cosolvent and 60Co as radiation source for gamma radiation
(10 kGy) to promote protein crosslinking. This technique promotes papain nanoparticle
sterilization and crosslinking in a single step showing that radiation is a promising technique for
the development of nanoparticles for biomedical use. However, important parameters such as the
influence of physical-chemical parameters over nanoparticle formation remains unexplored and
unclear. Therefore, this work aimed to evaluate the influence of pH and temperature over papain
nanoparticle formation including buffer molarity and different temperatures throughout the
synthesis and irradiation process. Nanopapain was synthetized as above-mentioned however
phosphate buffer was used in a range of 1 to 50 mM and ultrapure water in presence of ethanol
and irradiated at 10 kGy. The influence of temperature was evaluated by performing the synthesis
at -20, 0 and 20 °C. Additionally, it was verified the sterility of the material and its stability over
time. The hydrodynamic radius and crosslinking were evaluated by dynamic light scattering and
fluorescence measurements respectively. The pH range was influenced by buffer molarity, thus,
lower molarities led to a final pH of 5 and the higher molarities around 7. Bityrosine formation was
influenced by the pH range and increased when synthesis was performed near optimal pH (7 for
papain), although particle size didn’t undergo any changes as a result of the pH changes. On the
other hand, enzymatic activity was less affected by the process maintain about 80% of the native
enzymatic activity only when synthesis was carried out in 50 mM phosphate buffer. With regard
to temperature as the temperature increased the levels of crosslinking also increased in the order
of 20>0ºC>-20ºC. In conclusion nanoparticle size was not influenced by the temperature shift.
Concerning enzymatic activity samples irradiated under ice-cold conditions presented decreased
bioactivity loss if compared to the samples irradiated at room temperature. After an evaluation of
the results, the study revealed that samples synthetized with buffer molarity of 50 Mm (pH 7,2 ±
0,2) and 0ºC would promote higher crosslinking levels and less enzymatic activity decrease.
Como referenciar
FAZOLIN, GABRIELA N.; VARCA, GUSTAVO H.C.; LUGAO, ADEMAR B. The impact of physical-chemical parameters over the formation of papain nanoparticles crosslinked by radiation. In: PAN AMERICAN CONGRESS OF NANOTECHNOLOGY, 1st, 27-30 de novembro, 2017, Guarujá, SP. Abstract... Disponível em: http://repositorio.ipen.br/handle/123456789/29036. Acesso em: 20 Mar 2026.
Esta referência é gerada automaticamente de acordo com as normas do estilo IPEN/SP (ABNT NBR 6023) e recomenda-se uma verificação final e ajustes caso necessário.