Enhancement on the europium emission band of europium chlortetracycline complex in the presence of LDL
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Analytical Biochemistry
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Low-density lipoprotein (LDL) particles are the major cholesterol-carrying lipoprotein in the human circulation from the liver to peripheral tissues. High levels of LDL-Cholesterol (LDL-C) are known risk factor
for the development of coronary artery disease (CAD). The most common approach to determine the LDLC in the clinical laboratory involves the Friedewald formula. However, in certain situations, this approach
is inadequate. In this paper we report on the enhancement on the Europium emission band of Europium
chlortetracycline complex (CTEu) in the presence of LDL. The emission intensity at 615 nm of the CTEu
increases with increasing amounts of LDL. This phenomenon allowed us to propose a method to determine the LDL concentration in a sample composed by an aqueous solution of LDL. With this result we
obtained LDL calibration curve, LOD (limit of detection) of 0.49 mg/mL and SD (standard deviation) of
0.003. We observed that CTEu complex provides a wider dynamic concentration-range for LDL determination than that from Eu–tetracycline previously. The averaged emission lifetimes of the CTEu and CTEu
with LDL (1.5 mg/mL) complexes were measured as 15 and 46 ls, respectively. Study with some metallic
interferents is presented.
Como referenciar
TEIXEIRA, LUCIANE dos S.; GRASSO, ANDREA N.; MONTEIRO, ANDREA M.; FIGUEIREDO NETO, A.M.; VIEIRA JUNIOR, NILSON D.; GIDLUND, MAGNUS; COURROL, LILIA C. Enhancement on the europium emission band of europium chlortetracycline complex in the presence of LDL. Analytical Biochemistry, v. 400, n. 1, p. 19-24, 2010. DOI: 10.1016/j.ab.2010.01.030. Disponível em: http://repositorio.ipen.br/handle/123456789/4572. Acesso em: 20 Mar 2026.
Esta referência é gerada automaticamente de acordo com as normas do estilo IPEN/SP (ABNT NBR 6023) e recomenda-se uma verificação final e ajustes caso necessário.