Engineered papain nanoparticles by gamma irradiation: towards safe therapeutic applications

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dc.contributor.authorVARCA, GUSTAVO
dc.contributor.authorLIMA, MAYARA I.S.
dc.contributor.authorALVES, PATRICIA T.
dc.contributor.authorMOHAMED, LINA B.
dc.contributor.authorGRASSELLI, MARIANO
dc.contributor.authorGOULART, LUIZ R.
dc.contributor.authorLUGAO, ADEMAR B.
dc.coverageInternacionalpt_BR
dc.creator.eventoINTERNATIONAL MEETING ON RADIATION PROCESSING, 18thpt_BR
dc.date.accessioned2017-05-22T12:30:59Z
dc.date.available2017-05-22T12:30:59Z
dc.date.eventoNovember 07-11, 2016pt_BR
dc.description.abstractThis article provides an in vitro biological approach of the so called radiation synthesized protein-based nanoparticles as the use of nanoparticles for biomedical purposes requires precise understanding of their properties and effects on the body before clinical may take place. This technique allows proper control of papain nanoparticles with preserved biological activity for drug delivery. Biocompatibility results revealed that papain nanoparticles, of approximately 8-9 nm, were found to be biosafe in PBMC cells at concentrations up to 0.4 mg.ml-1 for up to 72 hours period. The nanoparticles were able to specifically bind to anti-Carica papaya polyclonal antibody with higher intensity than native papain. The stimuli of mouse splenocytes by papain nanoparticles led to the production of high levels of IL2. No significant levels of IL6, IL10 and IFNγ were produced by the stimuli. At nanoparticle concentrations above the cytotoxic limit, the concentration of IL2 was totally suppressed indicating no pro- nor anti-inflammatory response by means of cytokine mediated pathways. These in vitro results suggest that papain nanoparticles crosslinked by gamma irradiation may be safely administered with low nor negligible toxicity, holding strong applications for drug loading.pt_BR
dc.event.siglaIMRPpt_BR
dc.identifier.citationVARCA, GUSTAVO; LIMA, MAYARA I.S.; ALVES, PATRICIA T.; MOHAMED, LINA B.; GRASSELLI, MARIANO; GOULART, LUIZ R.; LUGAO, ADEMAR B. Engineered papain nanoparticles by gamma irradiation: towards safe therapeutic applications. In: INTERNATIONAL MEETING ON RADIATION PROCESSING, 18th, November 07-11, 2016, Vancouver, BC, Canada. <b>Abstract...</b> Disponível em: http://repositorio.ipen.br/handle/123456789/27479.
dc.identifier.orcidhttps://orcid.org/0000-0002-1737-3191
dc.identifier.urihttp://repositorio.ipen.br/handle/123456789/27479
dc.local.eventoVancouver, BC, Canadapt_BR
dc.rightsopenAccesspt_BR
dc.titleEngineered papain nanoparticles by gamma irradiation: towards safe therapeutic applicationspt_BR
dc.typeResumo de eventos científicospt_BR
dspace.entity.typePublication
ipen.autorLINA BADDIA MOHAMED
ipen.autorADEMAR BENEVOLO LUGAO
ipen.autorGUSTAVO HENRIQUE COSTAVARCA
ipen.codigoautor13998
ipen.codigoautor339
ipen.codigoautor8661
ipen.contributor.ipenauthorLINA BADDIA MOHAMED
ipen.contributor.ipenauthorADEMAR BENEVOLO LUGAO
ipen.contributor.ipenauthorGUSTAVO HENRIQUE COSTAVARCA
ipen.date.recebimento17-05pt_BR
ipen.event.datapadronizada2016pt_BR
ipen.identifier.ipendoc23777pt_BR
ipen.notas.internasAbstractpt_BR
ipen.type.genreResumo
relation.isAuthorOfPublication3f00c2ba-2b0d-4f0e-83e2-0138f7b29c16
relation.isAuthorOfPublication99ac24c5-2ae1-465a-a6f2-40b4d9af6af7
relation.isAuthorOfPublication46f998cf-b207-4854-94c0-dd2def4eec2a
relation.isAuthorOfPublication.latestForDiscovery46f998cf-b207-4854-94c0-dd2def4eec2a
sigepi.autor.atividadeVARCA, GUSTAVO:8661:750:Spt_BR
sigepi.autor.atividadeMOHAMED, LINA B.:13998:750:Npt_BR
sigepi.autor.atividadeLUGAO, ADEMAR B.:339:740:Npt_BR
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