High-level secretion of growth hormone by retrovirally transduced primary human keratinocytes

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dc.contributor.authorPERONI, CIBELE N.pt_BR
dc.contributor.authorCECCHI, CLAUDIA R.pt_BR
dc.contributor.authorDAMIANI, RENATApt_BR
dc.contributor.authorSOARES, CARLOS R.J.pt_BR
dc.contributor.authorRIBELA, MARIA T.C.P.pt_BR
dc.contributor.authorARKATEN, ROSANGELA R.pt_BR
dc.contributor.authorBARTOLINI, PAOLOpt_BR
dc.coverageInternacionalpt_BR
dc.date.accessioned2014-07-15T13:48:00Zpt_BR
dc.date.accessioned2014-07-30T11:51:15Z
dc.date.available2014-07-15T13:48:00Zpt_BR
dc.date.available2014-07-30T11:51:15Z
dc.date.issued2006pt_BR
dc.description.abstractA gene therapy clinical trial for treatment of growth hormone (GH) deficiency has not been reached yet, but several strategies using different gene transfer methodologies and animal models have been developed and showed successful results. We have set up an ex vivo gene therapy protocol using primary human keratinocytes transduced with an efficient retroviral vector (LXSN) encoding the human (hGH) or mouse GH (mGH) genes. These stably modified cells presented high in vitro expression levels of hGH (7 μg/106 cells/d) and mGH (11 μg/106 cells/d) after selection with geneticin. When the hGH-secreting keratinocytes were grafted onto immunodeficient dwarf mice (lit/scid), hGH levels in the circulation were about 0.2–0.3 ng/mL during a 12-d assay and these animals presented a significant body weight increase (p<0.01) compared to the control. Substitution of conventional grafting methodologies with organotypic raft cultures revealed a peak value of up to 20 ng mGH/mL in the circulation of grafted lit/scid mice at 1 h postimplantation, followed by a rapid decline to baseline (≈2 ng/mL) within 24 h. One week after grafting, however, the cultured excised implants still presented approx 45% of their original in vitro secretion efficiency. Further studies are being carrier out to identify the main factor(s) that still constitute one of the major impediments to the success of this promising model of cutaneous gene therapy.
dc.format.extent239-245pt_BR
dc.identifier.citationPERONI, CIBELE N.; CECCHI, CLAUDIA R.; DAMIANI, RENATA; SOARES, CARLOS R.J.; RIBELA, MARIA T.C.P.; ARKATEN, ROSANGELA R.; BARTOLINI, PAOLO. High-level secretion of growth hormone by retrovirally transduced primary human keratinocytes: prospects for an animal model of cutaneous gene therapy. <b>Molecular Biotechnology</b>, v. 34, n. 2, p. 239-245, 2006. DOI: <a href="https://dx.doi.org/10.1385/MB:34:2:239">10.1385/MB:34:2:239</a>. Disponível em: http://repositorio.ipen.br/handle/123456789/5426.
dc.identifier.doi10.1385/MB:34:2:239
dc.identifier.fasciculo2pt_BR
dc.identifier.issn1073-6085pt_BR
dc.identifier.orcidhttps://orcid.org/0000-0002-7982-1789
dc.identifier.orcidhttps://orcid.org/0000-0001-8194-5230
dc.identifier.urihttp://repositorio.ipen.br/handle/123456789/5426pt_BR
dc.identifier.vol34pt_BR
dc.relation.ispartofMolecular Biotechnologypt_BR
dc.rightsopenAccessen
dc.subjectsthpt_BR
dc.subjectkeratinpt_BR
dc.subjectgene therapypt_BR
dc.subjectepidermispt_BR
dc.subjectstem cellspt_BR
dc.subjectanimal cellspt_BR
dc.subjectsecretionpt_BR
dc.subjectmicept_BR
dc.subjectin vitropt_BR
dc.titleHigh-level secretion of growth hormone by retrovirally transduced primary human keratinocytespt_BR
dc.typeArtigo de periódicopt_BR
dspace.entity.typePublication
ipen.autorPAOLO BARTOLINI
ipen.autorROSANGELA DO ROCIO ARKATEN
ipen.autorMARIA TERESA DE CARVALHO PINTO RIBELA
ipen.autorCARLOS ROBERTO JORGE SOARES
ipen.autorRENATA DAMIANI
ipen.autorCLAUDIA REGINA CECCHI
ipen.autorCIBELE NUNES PERONI
ipen.codigoautor1503
ipen.codigoautor144
ipen.codigoautor1197
ipen.codigoautor509
ipen.codigoautor3931
ipen.codigoautor3865
ipen.codigoautor947
ipen.contributor.ipenauthorPAOLO BARTOLINI
ipen.contributor.ipenauthorROSANGELA DO ROCIO ARKATEN
ipen.contributor.ipenauthorMARIA TERESA DE CARVALHO PINTO RIBELA
ipen.contributor.ipenauthorCARLOS ROBERTO JORGE SOARES
ipen.contributor.ipenauthorRENATA DAMIANI
ipen.contributor.ipenauthorCLAUDIA REGINA CECCHI
ipen.contributor.ipenauthorCIBELE NUNES PERONI
ipen.date.recebimento06-12pt_BR
ipen.identifier.fi2.041pt_BR
ipen.identifier.ipendoc11548pt_BR
ipen.identifier.iwosWoSpt_BR
ipen.range.fi1.500 - 2.999
ipen.subtituloprospects for an animal model of cutaneous gene therapy
ipen.type.genreArtigo
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relation.isAuthorOfPublicationb30ee64c-1d00-47c8-b080-1467303aa384
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sigepi.autor.atividadePERONI, CIBELE N.:947:18:Spt_BR
sigepi.autor.atividadeCECCHI, CLAUDIA R.:3865:18:Npt_BR
sigepi.autor.atividadeDAMIANI, RENATA:3931:18:Npt_BR
sigepi.autor.atividadeSOARES, CARLOS R.J.:509:18:Npt_BR
sigepi.autor.atividadeRIBELA, MARIA T.C.P.:1197:18:Npt_BR
sigepi.autor.atividadeARKATEN, ROSANGELA R.:144:18:Npt_BR
sigepi.autor.atividadeBARTOLINI, PAOLO:1503:18:Npt_BR
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