Protein profile analysis of two australian snake venoms by one- dimensional gel electrophoresis and MS/MS experiments

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dc.contributor.authorGEORGIEVA, DESSISLAVA
dc.contributor.authorHILDEBRAND, DIANA
dc.contributor.authorSIMAS, RODRIGO
dc.contributor.authorCORONADO, MONIKA A.
dc.contributor.authorKWIATKOWSKI, MARCEL
dc.contributor.authorSCHLUTER, HARTMUT
dc.contributor.authorARNI, RAGHUVIR
dc.contributor.authorSPENCER, PATRICK
dc.contributor.authorBETZEL, CHRISTIAN
dc.coverageInternacionalpt_BR
dc.date.accessioned2017-09-18T11:30:32Z
dc.date.available2017-09-18T11:30:32Z
dc.date.issued2017pt_BR
dc.description.abstractThe Pseudechis colletti and Pseudechis butleri venoms were analyzed by 1-D gel electrophoresis, followed by mass spectrometric analysis of tryptic peptides obtained from the protein bands. Both venoms contain highly potent pharmacologically active components, which were assigned to the following protein families: basic and acidic phospholipases A2 (PLA2s), L-amino acid oxidases (LAAOs), P-III metalloproteinases (P-III SVMPs), 5’- nucleotidases (5’-NTDs), cysteine-rich secretory proteins (CRISPs), venom nerve growth factors (VNGFs) and post-synaptic neurotoxins. Considerable predominance of PLA2s over other toxins is a characteristic feature of both venoms. The major differences in the venom compositions are the higher concentration of SVMPs and CRISPs in the P. butleri venom, as well as the presence of post-synaptic neurotoxins. Furthermore, the analysis revealed a high concentration of proteins with myotoxic, coagulopathic and apoptotic activities. PLA2s are responsible for the myotoxic and anticoagulant effects observed in patients after envenomation (4). The other protein families, encountered in the two venoms, probably contribute to the major symptoms described for these venoms. These results explain the observed clinical effects of the black snake envenomation. The analyzed venoms contain group P-III metalloproteinases of medical importance with the potency to be used for diagnostic purposes of von Willebrand factor (vWF) disease, for regulation of vWF in thrombosis and haemostasis, for studying the function of the complement system in host defense and in the pathogenesis of diseases. Comparison of venomic data showed similarities in the major venom components of snakes from the genus Pseudechis, resulting in common clinical effects of envenomation, and demonstrating close relationships between venom toxins of Elapidae snakes.pt_BR
dc.format.extent1892-1908pt_BR
dc.identifier.citationGEORGIEVA, DESSISLAVA; HILDEBRAND, DIANA; SIMAS, RODRIGO; CORONADO, MONIKA A.; KWIATKOWSKI, MARCEL; SCHLUTER, HARTMUT; ARNI, RAGHUVIR; SPENCER, PATRICK; BETZEL, CHRISTIAN. Protein profile analysis of two australian snake venoms by one- dimensional gel electrophoresis and MS/MS experiments. <b>Current Medicinal Chemistry</b>, v. 24, n. 17, p. 1892-1908, 2017. DOI: <a href="https://dx.doi.org/10.2174/0929867324666170601073148">10.2174/0929867324666170601073148</a>. Disponível em: http://repositorio.ipen.br/handle/123456789/27743.
dc.identifier.doi10.2174/0929867324666170601073148pt_BR
dc.identifier.fasciculo17pt_BR
dc.identifier.issn1875-533Xpt_BR
dc.identifier.orcidhttps://orcid.org/0000-0001-8949-7735
dc.identifier.percentilfi71.09en
dc.identifier.urihttp://repositorio.ipen.br/handle/123456789/27743
dc.identifier.vol24pt_BR
dc.relation.ispartofCurrent Medicinal Chemistrypt_BR
dc.rightsclosedAccesspt_BR
dc.subjectproteins
dc.subjectprotein structure
dc.subjectsnakes
dc.subjectvenoms
dc.subjectelectrophoresis
dc.subjectpharmacology
dc.subjectmass spectroscopy
dc.subjectaustralia
dc.titleProtein profile analysis of two australian snake venoms by one- dimensional gel electrophoresis and MS/MS experimentspt_BR
dc.typeArtigo de periódicopt_BR
dspace.entity.typePublication
ipen.autorPATRICK JACK SPENCER
ipen.codigoautor910
ipen.contributor.ipenauthorPATRICK JACK SPENCER
ipen.date.recebimento17-09pt_BR
ipen.identifier.fi3.469pt_BR
ipen.identifier.ipendoc23048pt_BR
ipen.identifier.iwosWoSpt_BR
ipen.range.fi3.000 - 4.499
ipen.range.percentilfi50.00 - 74.99
ipen.type.genreArtigo
relation.isAuthorOfPublication4eb7939e-aeea-4991-8525-b3d05ac27364
relation.isAuthorOfPublication.latestForDiscovery4eb7939e-aeea-4991-8525-b3d05ac27364
sigepi.autor.atividadeSPENCER, PATRICK:910:820:Npt_BR
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