Can measurement of the fluorescence lifetime of extracted blood PPIX predict atherosclerosis?
dc.contributor.author | SICCHIERI, LETICIA B. | |
dc.contributor.author | SILVA, MONICA N. da | |
dc.contributor.author | SAMAD, RICARDO E. | |
dc.contributor.author | COURROL, LILIA C. | |
dc.coverage | Internacional | pt_BR |
dc.date.accessioned | 2018-04-18T13:01:45Z | |
dc.date.available | 2018-04-18T13:01:45Z | |
dc.date.issued | 2018 | pt_BR |
dc.description.abstract | In this work, fluorescence lifetime has been used to analyze protoporphyrin IX (PPIX) extracted from blood for diagnosing atherosclerosis. A total of 10 adult white male rabbits (New Zealand) were divided into the control group (CG), with a normal diet, and the experimental group (EG), subjected to a diet containing 1% cholesterol. Blood samples were collected from the animals, and protoporphyrin IX was extracted from the blood using acetone. The PPIX fluorescence lifetime (PPIXFL) was measured using time-correlated single photon counting, after excitation at 403 nm from a pulsed laser diode. It was found that the PPIX emission intensity was enhanced in the animals that had received a hypercholesterolemic diet. The CG and EG animal's fluorescence decays were fitted by three exponentials and the mean lifetimes were 4.0 ns and 9.5 ns, respectively. This lifetime dependence resulted in a calibration curve that allows the determination of the PPIX concentration with a temporal measurement. The obtained results show that fluorescence lifetime can potentially be used as a noninvasive, simple, rapid, and sensitive tool in atherosclerosis diagnosis. | pt_BR |
dc.description.sponsorship | Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) | pt_BR |
dc.description.sponsorshipID | FAPESP: 14/06960-9 | pt_BR |
dc.format.extent | 176-180 | pt_BR |
dc.identifier.citation | SICCHIERI, LETICIA B.; SILVA, MONICA N. da; SAMAD, RICARDO E.; COURROL, LILIA C. Can measurement of the fluorescence lifetime of extracted blood PPIX predict atherosclerosis?. <b>Journal of Luminescence</b>, v. 195, p. 176-180, 2018. DOI: <a href="https://dx.doi.org/10.1016/j.jlumin.2017.11.014">10.1016/j.jlumin.2017.11.014</a>. Disponível em: http://repositorio.ipen.br/handle/123456789/28855. | |
dc.identifier.doi | 10.1016/j.jlumin.2017.11.014 | pt_BR |
dc.identifier.issn | 0022-2313 | pt_BR |
dc.identifier.orcid | https://orcid.org/0000-0001-7762-8961 | |
dc.identifier.percentilfi | 73.16 | en |
dc.identifier.percentilfiCiteScore | 73.60 | |
dc.identifier.uri | http://repositorio.ipen.br/handle/123456789/28855 | |
dc.identifier.vol | 195 | pt_BR |
dc.relation.ispartof | Journal of Luminescence | pt_BR |
dc.rights | openAccess | pt_BR |
dc.subject | arteriosclerosis | |
dc.subject | protoporphyrins | |
dc.subject | fluorescence spectroscopy | |
dc.subject | blood | |
dc.subject | bioassay | |
dc.subject | lifetime | |
dc.subject | diagnostic uses | |
dc.subject | diagnosis | |
dc.title | Can measurement of the fluorescence lifetime of extracted blood PPIX predict atherosclerosis? | pt_BR |
dc.type | Artigo de periódico | pt_BR |
dspace.entity.type | Publication | |
ipen.autor | LETICIA BONFANTE SICCHIERI | |
ipen.autor | LILIA CORONATO COURROL | |
ipen.autor | RICARDO ELGUL SAMAD | |
ipen.codigoautor | 9318 | |
ipen.codigoautor | 1750 | |
ipen.codigoautor | 909 | |
ipen.contributor.ipenauthor | LETICIA BONFANTE SICCHIERI | |
ipen.contributor.ipenauthor | LILIA CORONATO COURROL | |
ipen.contributor.ipenauthor | RICARDO ELGUL SAMAD | |
ipen.date.recebimento | 18-04 | pt_BR |
ipen.identifier.fi | 2.961 | pt_BR |
ipen.identifier.fiCiteScore | 4.8 | |
ipen.identifier.ipendoc | 24328 | pt_BR |
ipen.identifier.iwos | WoS | pt_BR |
ipen.range.fi | 1.500 - 2.999 | |
ipen.range.percentilfi | 50.00 - 74.99 | |
ipen.type.genre | Artigo | |
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sigepi.autor.atividade | SICCHIERI, LETICIA B.:9318:-1:S | pt_BR |
sigepi.autor.atividade | SAMAD, RICARDO E.:909:930:N | pt_BR |
sigepi.autor.atividade | COURROL, LILIA C.:1750:920:N | pt_BR |