Can measurement of the fluorescence lifetime of extracted blood PPIX predict atherosclerosis?

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dc.contributor.authorSICCHIERI, LETICIA B.
dc.contributor.authorSILVA, MONICA N. da
dc.contributor.authorSAMAD, RICARDO E.
dc.contributor.authorCOURROL, LILIA C.
dc.coverageInternacionalpt_BR
dc.date.accessioned2018-04-18T13:01:45Z
dc.date.available2018-04-18T13:01:45Z
dc.date.issued2018pt_BR
dc.description.abstractIn this work, fluorescence lifetime has been used to analyze protoporphyrin IX (PPIX) extracted from blood for diagnosing atherosclerosis. A total of 10 adult white male rabbits (New Zealand) were divided into the control group (CG), with a normal diet, and the experimental group (EG), subjected to a diet containing 1% cholesterol. Blood samples were collected from the animals, and protoporphyrin IX was extracted from the blood using acetone. The PPIX fluorescence lifetime (PPIXFL) was measured using time-correlated single photon counting, after excitation at 403 nm from a pulsed laser diode. It was found that the PPIX emission intensity was enhanced in the animals that had received a hypercholesterolemic diet. The CG and EG animal's fluorescence decays were fitted by three exponentials and the mean lifetimes were 4.0 ns and 9.5 ns, respectively. This lifetime dependence resulted in a calibration curve that allows the determination of the PPIX concentration with a temporal measurement. The obtained results show that fluorescence lifetime can potentially be used as a noninvasive, simple, rapid, and sensitive tool in atherosclerosis diagnosis.pt_BR
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)pt_BR
dc.description.sponsorshipIDFAPESP: 14/06960-9pt_BR
dc.format.extent176-180pt_BR
dc.identifier.citationSICCHIERI, LETICIA B.; SILVA, MONICA N. da; SAMAD, RICARDO E.; COURROL, LILIA C. Can measurement of the fluorescence lifetime of extracted blood PPIX predict atherosclerosis?. <b>Journal of Luminescence</b>, v. 195, p. 176-180, 2018. DOI: <a href="https://dx.doi.org/10.1016/j.jlumin.2017.11.014">10.1016/j.jlumin.2017.11.014</a>. Disponível em: http://repositorio.ipen.br/handle/123456789/28855.
dc.identifier.doi10.1016/j.jlumin.2017.11.014pt_BR
dc.identifier.issn0022-2313pt_BR
dc.identifier.orcidhttps://orcid.org/0000-0001-7762-8961
dc.identifier.percentilfi73.16en
dc.identifier.percentilfiCiteScore73.60
dc.identifier.urihttp://repositorio.ipen.br/handle/123456789/28855
dc.identifier.vol195pt_BR
dc.relation.ispartofJournal of Luminescencept_BR
dc.rightsopenAccesspt_BR
dc.subjectarteriosclerosis
dc.subjectprotoporphyrins
dc.subjectfluorescence spectroscopy
dc.subjectblood
dc.subjectbioassay
dc.subjectlifetime
dc.subjectdiagnostic uses
dc.subjectdiagnosis
dc.titleCan measurement of the fluorescence lifetime of extracted blood PPIX predict atherosclerosis?pt_BR
dc.typeArtigo de periódicopt_BR
dspace.entity.typePublication
ipen.autorLETICIA BONFANTE SICCHIERI
ipen.autorLILIA CORONATO COURROL
ipen.autorRICARDO ELGUL SAMAD
ipen.codigoautor9318
ipen.codigoautor1750
ipen.codigoautor909
ipen.contributor.ipenauthorLETICIA BONFANTE SICCHIERI
ipen.contributor.ipenauthorLILIA CORONATO COURROL
ipen.contributor.ipenauthorRICARDO ELGUL SAMAD
ipen.date.recebimento18-04pt_BR
ipen.identifier.fi2.961pt_BR
ipen.identifier.fiCiteScore4.8
ipen.identifier.ipendoc24328pt_BR
ipen.identifier.iwosWoSpt_BR
ipen.range.fi1.500 - 2.999
ipen.range.percentilfi50.00 - 74.99
ipen.type.genreArtigo
relation.isAuthorOfPublication99f51182-5568-4fe2-8f29-5da85b3877f9
relation.isAuthorOfPublication342c837c-9558-4c43-bb2d-846bab821ad6
relation.isAuthorOfPublicationb9c43c0c-87a6-4ebd-92ff-2515c4ac1d34
relation.isAuthorOfPublication.latestForDiscovery99f51182-5568-4fe2-8f29-5da85b3877f9
sigepi.autor.atividadeSICCHIERI, LETICIA B.:9318:-1:Spt_BR
sigepi.autor.atividadeSAMAD, RICARDO E.:909:930:Npt_BR
sigepi.autor.atividadeCOURROL, LILIA C.:1750:920:Npt_BR
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