Paratrygon aiereba irradiated anti-mucus serum reduce edematogenic activity induced in experimental model

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dc.contributor.authorTHOMAZI, GABRIELA O.C.pt_BR
dc.contributor.authorCOSTA, ANDREA dapt_BR
dc.contributor.authorRODRIGUES, JAQUELINE P.pt_BR
dc.contributor.authorALVES, GLAUCIE J.pt_BR
dc.contributor.authorPREZOTTO NETO, JOSE P.pt_BR
dc.contributor.authorTURIBIO, THOMPSON de O.pt_BR
dc.contributor.authorROCHA, ANDRE M.pt_BR
dc.contributor.authorAIRES, RAQUEL da S.pt_BR
dc.contributor.authorSEIBERT, CARLA S.pt_BR
dc.contributor.authorSPENCER, PATRICK J.pt_BR
dc.contributor.authorGALISTEO JUNIOR, ANDRES J.pt_BR
dc.contributor.authorANDRADE JUNIOR, HEITOR F. dept_BR
dc.contributor.authorNASCIMENTO, NANCI dopt_BR
dc.coverageInternacionalpt_BR
dc.date.accessioned2020-10-16T20:06:16Z
dc.date.available2020-10-16T20:06:16Z
dc.date.issued2020pt_BR
dc.description.abstractAccidents by freshwater stingrays are common in northern Brazil, there is no specific therapy for high morbidity and local tissue destruction. The irradiation of venoms and toxins by ionizing radiation has been used to produce appropriate immunogens for the production of antisera. We planned to study the efficacy of stinging mucus irradiation in the production of antisera, with serum neutralization assays of edematogenic activity and quantification of cytokines performed in animal models of immunization with native and irradiated mucus of Paratrygon aiereba, a large freshwater stingray. Antiserum potency and its cross-reactivity with mucus from other freshwater stingrays were detected by ELISA. Immunization models demonstrated the ability to stimulate a strong humoral response with elevated levels of serum IgG detectable by ELISA, and both native and irradiated mucus were immunogenic and capable of recognizing mucus proteins from other freshwater neotropical stingrays. Mucus P. aiereba causes cellular and humoral adaptive immune responses in cells of immunized mice producing antibodies and cytokines such as TNF-α, IL-6 and IL-17. Rabbit antisera immunized with mucus from P. aiereba irradiated at 2 kGy showed a significant reduction of mucus-induced edematogenic activity in mice. Our data suggest that the use of antisera against freshwater stingray mucus show the possibility of specific therapy for these accidents.pt_BR
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)pt_BR
dc.description.sponsorshipIDCNPq :457366/2014pt_BR
dc.format.extent13-19pt_BR
dc.identifier.citationTHOMAZI, GABRIELA O.C.; COSTA, ANDREA da; RODRIGUES, JAQUELINE P.; ALVES, GLAUCIE J.; PREZOTTO NETO, JOSE P.; TURIBIO, THOMPSON de O.; ROCHA, ANDRE M.; AIRES, RAQUEL da S.; SEIBERT, CARLA S.; SPENCER, PATRICK J.; GALISTEO JUNIOR, ANDRES J.; ANDRADE JUNIOR, HEITOR F. de; NASCIMENTO, NANCI do. Paratrygon aiereba irradiated anti-mucus serum reduce edematogenic activity induced in experimental model. <b>Toxicon</b>, v. 178, p. 13-19, 2020. DOI: <a href="https://dx.doi.org/10.1016/j.toxicon.2020.02.012">10.1016/j.toxicon.2020.02.012</a>. Disponível em: http://repositorio.ipen.br/handle/123456789/31450.
dc.identifier.doi10.1016/j.toxicon.2020.02.012pt_BR
dc.identifier.issn0041-0101pt_BR
dc.identifier.orcid0000-0001-8949-7735pt_BR
dc.identifier.orcidhttps://orcid.org/0000-0001-8949-7735
dc.identifier.percentilfi40.26pt_BR
dc.identifier.percentilfiCiteScore39.00
dc.identifier.urihttp://repositorio.ipen.br/handle/123456789/31450
dc.identifier.vol178pt_BR
dc.relation.ispartofToxiconpt_BR
dc.rightsopenAccesspt_BR
dc.subjectfishes
dc.subjectvenoms
dc.subjecttoxicity
dc.subjectimmunity
dc.subjectimmunoassay
dc.subjectgamma radiation
dc.subjectimmune serums
dc.subjectenzyme immunoassay
dc.subjectfresh water
dc.subjectaquatic ecosystems
dc.subjectantibodies
dc.subjectionizing radiations
dc.titleParatrygon aiereba irradiated anti-mucus serum reduce edematogenic activity induced in experimental modelpt_BR
dc.typeArtigo de periódicopt_BR
dspace.entity.typePublication
ipen.autorGLAUCIE JUSSILANE ALVES
ipen.autorTHOMPSON DE OLIVEIRA TURIBIO
ipen.autorRAQUEL DA SILVA AIRES
ipen.autorNANCI DO NASCIMENTO
ipen.autorPATRICK JACK SPENCER
ipen.autorANDRE MOREIRA ROCHA
ipen.autorJOSE PEDRO PREZOTTO NETO
ipen.autorGABRIELA ORTEGA COELHO THOMAZI
ipen.codigoautor12131
ipen.codigoautor12106
ipen.codigoautor12086
ipen.codigoautor411
ipen.codigoautor910
ipen.codigoautor12079
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ipen.codigoautor12056
ipen.contributor.ipenauthorGLAUCIE JUSSILANE ALVES
ipen.contributor.ipenauthorTHOMPSON DE OLIVEIRA TURIBIO
ipen.contributor.ipenauthorRAQUEL DA SILVA AIRES
ipen.contributor.ipenauthorNANCI DO NASCIMENTO
ipen.contributor.ipenauthorPATRICK JACK SPENCER
ipen.contributor.ipenauthorANDRE MOREIRA ROCHA
ipen.contributor.ipenauthorJOSE PEDRO PREZOTTO NETO
ipen.contributor.ipenauthorGABRIELA ORTEGA COELHO THOMAZI
ipen.date.recebimento20-10
ipen.identifier.fi3.033pt_BR
ipen.identifier.fiCiteScore3.4
ipen.identifier.ipendoc27224pt_BR
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ipen.type.genreArtigo
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sigepi.autor.atividadeNASCIMENTO, NANCI do:411:-1:Npt_BR
sigepi.autor.atividadeSPENCER, PATRICK J.:910:810:Npt_BR
sigepi.autor.atividadeAIRES, RAQUEL da S.:12086:830:Npt_BR
sigepi.autor.atividadeROCHA, ANDRE M.:12079:-1:Npt_BR
sigepi.autor.atividadeTURIBIO, THOMPSON de O.:12106:830:Npt_BR
sigepi.autor.atividadePREZOTTO NETO, JOSE P.:12680:810:Npt_BR
sigepi.autor.atividadeALVES, GLAUCIE J.:12131:810:Npt_BR
sigepi.autor.atividadeTHOMAZI, GABRIELA O.C.:12056:-1:Spt_BR
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