Isolation and partial characterization of 3 nontoxic D‐galactose– specific isolectins from seeds of Momordica balsamina
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Journal of Molecular Recognition
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Three isolectins denoted hereforth MBaL‐30, MBaL‐60, and MBaL‐80 were isolated from seeds
extract of Momordica balsamina by 30%, 60%, and 80% ammonium sulfate saturations, respectively.
The native molecular weights of these lectins, as judged by gel filtration, were 108, 56,
and 160 kDa, respectively. On SDS‐PAGE, under reduced condition, 27 kDa band was obtained
for all isolectins. The lectins hemagglutinating activities were variably inhibited by D‐galactose
(minimum inhibitory concentrations = 12.5mM, 50mM, and 0.391mM, respectively). MBaL‐30
and ‐60 could agglutinate all human blood types with slight preference for the A and O blood
groups, whereas MBaL‐80 did not agglutinate B and AB blood types. The 3 isolectins were purified
from crude seeds extract, collectively, in a single step on the affinity matrix Lactamyl‐Seralose
4B; this purified lectin fraction, which contains all isolectins, is termed MBaL. The N‐terminal of
MBaL till the 25th amino acid was NLSLSELDFSADTYKSFIKNLRKQL, which shares 88%
sequence identity with Momordica charantia lectin type‐2 ribosomal inactivating protein from
Momordica charantia and 50% with momordin II from Momordica balsamina. MBaL retained
100% activity at up to 50°C for 30 minutes. MBaL‐30 and MBaL‐60 exhibited maximum activities
in the pH range between 4 and 8, while MBaL‐80 was showing maximum activity in the pH range
between 3 and 5. Treatment of MBaL‐30 and MBaL‐60 with EDTA completely abolished their
hemagglutinating activities. Addition of Zn and Fe ions to the ethylenediaminetetraacetic acid–
treated MBaL‐30 and MBaL‐60 lectins did not only regained the loss of activity but also resulted
in 200% to 300% increase in activity, respectively. MBaL‐30 and ‐60 agglutinated gram positive
Listeria monocytogenes and Staphylococcus aureus, whereas MBaL‐30 could merely agglutinate
Escherichia coli. None of these lectins could arrest bacterial growth. Addition of MBaL to cancer
cell lines (Gastric cancer cell line (AGS) and Gastric cencer cell line (MKN45), Glioblastoma
(ECV‐304), and Human urinary bladder cancer cell line (U87‐MG)) at varying concentrations did
not cause statistically significant changes on cell growth and viability.
Como referenciar
AWADALLAH, AMNA K.E.; OSMAN, MAKARIM E.M.; IBRAHIM, MARIAM A.; BERNARDES, EMERSON S.; DIAS-BARUFFI, MARCELO; KONOZY, EMADELDIN H.E. Isolation and partial characterization of 3 nontoxic D‐galactose– specific isolectins from seeds of Momordica balsamina. Journal of Molecular Recognition, v. 30, n. 2, p. 1-12, 2017. DOI: 10.1002/jmr.2582. Disponível em: http://repositorio.ipen.br/handle/123456789/27372. Acesso em: 30 Dec 2025.
Esta referência é gerada automaticamente de acordo com as normas do estilo IPEN/SP (ABNT NBR 6023) e recomenda-se uma verificação final e ajustes caso necessário.