Microwave-mediated synthesis of iron-oxide nanoparticles for use in magnetic levitation cell cultures

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dc.contributor.authorBONFIM, LETICIApt_BR
dc.contributor.authorPASSOS, PRISCILA de Q.S.pt_BR
dc.contributor.authorGONÇALVES, KARINA de O.pt_BR
dc.contributor.authorCOURROL, LILIA C.pt_BR
dc.contributor.authorSILVA, FLAVIA R. de O.pt_BR
dc.contributor.authorVIEIRA, DANIEL P.pt_BR
dc.coverageInternacionalpt_BR
dc.date.accessioned2020-02-21T11:58:31Z
dc.date.available2020-02-21T11:58:31Z
dc.date.issued2019pt_BR
dc.description.abstractThe use of three-dimensional cell cultures has been widely used for efficacy and/or toxicity testing of compounds. One of the most promising systems, based on magnetic levitation, is dependent on proper cell magnetization, achieved through adsorption of iron-oxide nanoparticles on cell membranes. These particles must bare not only significant responses to magnetic fields, but also a stable mechanism to attachment to cells. This work proposes a simple, one-pot synthesis method to produce magnetite nanoparticles, using a Fe2+ precursor associated with amino acids under microwave heating, and successive steps to confer positive charges to particles. X-ray diffraction could confirm Fe3O4 composition, and TEM analysis showed cubic-like crystallites with less than 50 nm. Zeta-potential experiments showed that particles remained positively charged (20.98 ± 0.28 mV) in physiological pH, suggesting ability to attach to (negatively charged) cell membranes, observed through optical microscopy. Iron colloid was found to be non-cytotoxic in concentrations up to 8% in cell culture media. Finally, human prostate cancer cells were cultured in 96-well plates using magnetic levitation and could be kept 8 days in culture. The results showed a feasible way to produce spheroids relying on magnetic levitation, using a newly described method of magnetic and cell adherent nanoparticle production.pt_BR
dc.format.extent1707-1717pt_BR
dc.identifier.citationBONFIM, LETICIA; PASSOS, PRISCILA de Q.S.; GONÇALVES, KARINA de O.; COURROL, LILIA C.; SILVA, FLAVIA R. de O.; VIEIRA, DANIEL P. Microwave-mediated synthesis of iron-oxide nanoparticles for use in magnetic levitation cell cultures. <b>Applied Nanoscience</b>, v. 9, n. 8, p. 1707-1717, 2019. DOI: <a href="https://dx.doi.org/10.1007/s13204-019-00962-1">10.1007/s13204-019-00962-1</a>. Disponível em: http://repositorio.ipen.br/handle/123456789/30817.
dc.identifier.doi10.1007/s13204-019-00962-1pt_BR
dc.identifier.fasciculo8pt_BR
dc.identifier.issn2190-5509pt_BR
dc.identifier.orcid0000-0002-0007-534Xpt_BR
dc.identifier.orcidhttps://orcid.org/0000-0002-0007-534X
dc.identifier.percentilfi44.175pt_BR
dc.identifier.percentilfiCiteScore71.67
dc.identifier.urihttp://repositorio.ipen.br/handle/123456789/30817
dc.identifier.vol9pt_BR
dc.relation.ispartofApplied Nanosciencept_BR
dc.rightsopenAccesspt_BR
dc.subjectnanotechnology
dc.subjectiron oxides
dc.subjectmicrowave equipment
dc.subjectsynthesis
dc.subjectnanoparticles
dc.subjectiron oxides
dc.subjectparamagnetism
dc.subjecttoxicity
dc.subjectx-ray diffraction
dc.titleMicrowave-mediated synthesis of iron-oxide nanoparticles for use in magnetic levitation cell culturespt_BR
dc.typeArtigo de periódicopt_BR
dspace.entity.typePublication
ipen.autorPRISCILA DE QUEIROZ SOUZA PASSOS
ipen.autorDANIEL PEREZ VIEIRA
ipen.autorFLAVIA RODRIGUES DE OLIVEIRA SILVA
ipen.autorLETICIA BONFIM
ipen.codigoautor14722
ipen.codigoautor3158
ipen.codigoautor5910
ipen.codigoautor14185
ipen.contributor.ipenauthorPRISCILA DE QUEIROZ SOUZA PASSOS
ipen.contributor.ipenauthorDANIEL PEREZ VIEIRA
ipen.contributor.ipenauthorFLAVIA RODRIGUES DE OLIVEIRA SILVA
ipen.contributor.ipenauthorLETICIA BONFIM
ipen.date.recebimento20-02
ipen.identifier.fi2.880pt_BR
ipen.identifier.fiCiteScore4.9
ipen.identifier.ipendoc26645pt_BR
ipen.identifier.iwosWoSpt_BR
ipen.identifier.ods3
ipen.range.fi1.500 - 2.999
ipen.range.percentilfi25.00 - 49.99
ipen.type.genreArtigo
relation.isAuthorOfPublicationbe35124f-007e-45eb-ba9d-2eba9d9b176d
relation.isAuthorOfPublicationc2352608-be9c-4a73-be8c-571f10bb53d2
relation.isAuthorOfPublicationb1aa6134-5ebc-46f0-ada3-605eaec3a986
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relation.isAuthorOfPublication.latestForDiscovery4cddf0c8-9b2f-4ec1-9621-8fbf918f0b3f
sigepi.autor.atividadeVIEIRA, DANIEL P.:3158:810:Npt_BR
sigepi.autor.atividadeSILVA, FLAVIA R. de O.:5910:820:Npt_BR
sigepi.autor.atividadePASSOS, PRISCILA de Q.S.:14722:810:Npt_BR
sigepi.autor.atividadeBONFIM, LETICIA:14185:810:Spt_BR
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