Effect of implant cleaning on titanium particle dissolution and cytocompatibility

Página do item simplificado
dc.contributor.authorKOTSAKIS, GEORGIOS A.pt_BR
dc.contributor.authorBLACK, RACHELpt_BR
dc.contributor.authorKUM, JASONpt_BR
dc.contributor.authorBERBEL, LARISSApt_BR
dc.contributor.authorSADR, ALIpt_BR
dc.contributor.authorKAROUSSIS, IOANNISpt_BR
dc.contributor.authorSIMOPOULOU, MARApt_BR
dc.contributor.authorDAUBERT, DIANEpt_BR
dc.coverageInternacionalpt_BR
dc.date.accessioned2021-07-27T13:24:03Z
dc.date.available2021-07-27T13:24:03Z
dc.date.issued2021pt_BR
dc.description.abstractBackground Peri-implantitis treatments are mainly based on protocols for teeth but have not shown favorable outcomes for implants. The potential role of titanium dissolution products in peri-implantitis necessitate the consideration of material properties in devising treatment protocols. We assessed implant cleaning interventions on (1) bacterial removal from Ti-bound biofilms, (2) Ti surface alterations and related Ti particle dissolution, and (3) cytocompatibility. Methods Acid-etched Ti discs were inoculated with human peri-implant plaque biofilms and mechanical antimicrobial interventions were applied on the Ti-bound biofilms for 30 seconds each: (1) rotary nylon brush; (2) Ti brush; (3) water-jet on high and (4) low, and compared to sterile, untreated and Chlorhexidine-treated controls. We assessed colony forming units (CFU) counts, biofilm removal, surface changes via scanning electron microscopy (SEM) and atomic force microscopy (AFM), and Ti dissolution via light microscopy and Inductively-coupled Mass Spectrometry (ICP-MS). Biological effects of Ti particles and surfaces changes were assessed using NIH/3T3 fibroblasts and MG-63 osteoblastic cell lines, respectively. Results Sequencing revealed that the human biofilm model supported a diverse biofilm including known peri-implant pathogens. WJ and Nylon brush were most effective in reducing CFU counts (P < 0.01 versus control), whereas Chlorhexidine was least effective; biofilm imaging results were confirmatory. Ti brushes led to visible streaks on the treated surfaces, reduced corrosion resistance and increased Ti dissolution over 30 days of material aging as compared to controls, which increase was amplified in the presence of bacteria (all P-val < 0.05). Ti particles exerted cytotoxic effects against fibroblasts, whereas surfaces altered by Ti brushes exhibited reduced osteoconductivity versus controls (P < 0.05). Conclusions Present findings support that mechanical treatment strategies selected for implant biofilm removal may lead to Ti dissolution. Ti dissolution should become an important consideration in the clinical selection of peri-implantitis treatments and a necessary criterion for the regulatory approval of instruments for implant hygiene.pt_BR
dc.format.extent580-591pt_BR
dc.identifier.citationKOTSAKIS, GEORGIOS A.; BLACK, RACHEL; KUM, JASON; BERBEL, LARISSA; SADR, ALI; KAROUSSIS, IOANNIS; SIMOPOULOU, MARA; DAUBERT, DIANE. Effect of implant cleaning on titanium particle dissolution and cytocompatibility. <b>Journal of Periodontology</b>, v. 92, n. 4, p. 580-591, 2021. DOI: <a href="https://dx.doi.org/10.1002/JPER.20-0186">10.1002/JPER.20-0186</a>. Disponível em: http://repositorio.ipen.br/handle/123456789/32062.
dc.identifier.doi10.1002/JPER.20-0186pt_BR
dc.identifier.fasciculo4pt_BR
dc.identifier.issn0022-3492pt_BR
dc.identifier.percentilfi87.50pt_BR
dc.identifier.percentilfiCiteScore80.00
dc.identifier.urihttp://repositorio.ipen.br/handle/123456789/32062
dc.identifier.vol92pt_BR
dc.relation.ispartofJournal of Periodontologypt_BR
dc.rightsclosedAccesspt_BR
dc.subjectdentistry
dc.subjectimplants
dc.subjecttitanium
dc.subjectbacteria
dc.subjectcorrosion
dc.subjectsurface properties
dc.subjectantimicrobial agents
dc.titleEffect of implant cleaning on titanium particle dissolution and cytocompatibilitypt_BR
dc.typeArtigo de periódicopt_BR
dspace.entity.typePublication
ipen.autorLARISSA OLIVEIRA BERBEL
ipen.codigoautor14612
ipen.contributor.ipenauthorLARISSA OLIVEIRA BERBEL
ipen.date.recebimento21-07
ipen.identifier.fi4.494pt_BR
ipen.identifier.fiCiteScore10.5
ipen.identifier.ipendoc27833pt_BR
ipen.identifier.iwosWoSpt_BR
ipen.range.fi3.000 - 4.499
ipen.range.percentilfi75.00 - 100.00
ipen.type.genreArtigo
relation.isAuthorOfPublication3c19a0f9-929f-43b8-a9ef-3319a0e34525
relation.isAuthorOfPublication.latestForDiscovery3c19a0f9-929f-43b8-a9ef-3319a0e34525
sigepi.autor.atividadeBERBEL, LARISSA:14612:730:Npt_BR
Pacote Original
Agora exibindo 1 - 1 de 1
Nenhuma Miniatura disponível
Nome:
27833.pdf
Tamanho:
4.89 MB
Formato:
Adobe Portable Document Format
Descrição:
Baixar
Licença do Pacote
Agora exibindo 1 - 1 de 1
Nenhuma Miniatura disponível
Nome:
license.txt
Tamanho:
1.71 KB
Formato:
Item-specific license agreed upon to submission
Descrição:
Baixar
Coleções
Periódicos