Biochemical and biological characterization of the Hypanus americanus mucus
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2019
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Fish and Shellfish Immunology
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Stingrays skin secretions are largely studied due to the human envenoming medical relevance of the sting
puncture that evolves to inflammatory events, including necrosis. Such toxic effects can be correlated to the
biochemical composition of the sting mucus, according to the literature. Fish skin plays important biological
roles, such as the control of the osmotic pressure gradient, protection against mechanical forces and microorganism
infections. The mucus, on the other hand, is a rich and complex fluid, acting on swimming, nutrition
and the innate immune system. The elasmobranch's epidermis is a tissue composed mainly by mucus secretory
cells, and marine stingrays have already been described to present secretory glands spread throughout the body.
Little is known about the biochemical composition of the stingray mucus, but recent studies have corroborated
the importance of mucus in the envenomation process. Aiming to assess the mucus composition, a new noninvasive
mucus collection method was developed that focused on peptides and proteins, and biological assays
were performed to analyze the toxic and immune activities of the Hypanus americanus mucus. Pathophysiological
characterization showed the presence of peptidases on the mucus, as well as the induction of edema and leukocyte
recruitment in mice. The fractionated mucus improved phagocytosis on macrophages and showed antimicrobial
activity against T. rubrumç. neoformans and C. albicans in vitro. The proteomic analyses showed the
presence of immune-related proteins like actin, histones, hemoglobin, and ribosomal proteins. This protein
pattern is similar to those reported for other fish mucus and stingray venoms. This is the first report depicting the
Hypanus stingray mucus composition, highlighting its biochemical composition and importance for the stingray
immune system and the possible role on the envenomation process.
Como referenciar
COELHO, GUILHERME R.; PREZOTTO NETO, PEDRO; BARBOSA, FERNANDA C.; SANTOS, RAFAEL S. dos; BRIGATTE, PATRICIA; SPENCER, PATRICK J.; SAMPAIO, SANDRA C.; D’AMELIO, FERNANDA; PIMENTA, DANIEL C.; SCIANI, JULIANA M. Biochemical and biological characterization of the Hypanus americanus mucus: a perspective on stingray immunity and toxins. Fish and Shellfish Immunology, v. 93, p. 832-840, 2019. DOI: 10.1016/j.fsi.2019.08.049. Disponível em: http://repositorio.ipen.br/handle/123456789/30383. Acesso em: 16 Sep 2024.
Esta referência é gerada automaticamente de acordo com as normas do estilo IPEN/SP (ABNT NBR 6023) e recomenda-se uma verificação final e ajustes caso necessário.