Species-specific in vitro and in vivo evaluation of toxicity of silver nanoparticles stabilized with gum arabic protein

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dc.contributor.authorMAZIERO, JOANA S.pt_BR
dc.contributor.authorTHIPE, VELAPHI C.pt_BR
dc.contributor.authorROGERO, SIZUE O.pt_BR
dc.contributor.authorCAVALCANTE, ADRIANA K.pt_BR
dc.contributor.authorDAMASCENO, KELME C.pt_BR
dc.contributor.authorORMENIO, MATHEUS B.pt_BR
dc.contributor.authorMARTINI, GISELA A.pt_BR
dc.contributor.authorBATISTA, JORGE G.S.pt_BR
dc.contributor.authorVIVEIROS, WILLIAMpt_BR
dc.contributor.authorKATTI, KAVITA K.pt_BR
dc.contributor.authorKARIKACHERY, ALICE R.pt_BR
dc.contributor.authorMOHANDOSS, DARSHAKUMAR D.pt_BR
dc.contributor.authorDHURVAS, RASHMI D.pt_BR
dc.contributor.authorNAPPINNAI, MOHANAVELUpt_BR
dc.contributor.authorROGERO, JOSE R.pt_BR
dc.contributor.authorLUGAO, ADEMAR B.pt_BR
dc.contributor.authorKATTI, KATTESH V.pt_BR
dc.coverageInternacionalpt_BR
dc.date.accessioned2020-12-09T13:50:30Z
dc.date.available2020-12-09T13:50:30Z
dc.date.issued2020pt_BR
dc.description.abstractIntroduction: We report, herein, in vitro, and in vivo toxicity evaluation of silver nanoparticles stabilized with gum arabic protein (AgNP-GP) in Daphnia similis, Danio rerio embryos and in Sprague Dawley rats. Purpose: The objective of this investigation was to evaluate in vitro and in vivo toxicity of silver nanoparticles stabilized with gum arabic protein (AgNP-GP), in multispecies due to the recognition that toxicity evaluations beyond a single species reflect the environmental realism. In the present study, AgNP-GP was synthesized through the reduction of silver salt using the tri-alanine-phosphine peptide (commonly referred to as “Katti Peptide”) and stabilized using gum arabic protein. Methods: In vitro cytotoxicity tests were performed according to ISO 10993– 5 protocols to assess cytotoxicity index (IC50) values. Acute ecotoxicity (EC50) studies were performed using Daphnia similis, according to the ABNT NBR 15088 protocols. In vivo toxicity also included evaluation of acute embryotoxicity using Danio rerio (zebrafish) embryos following the OECD No. 236 guidelines. We also used Sprague Dawley rats to assess the toxicity of AgNP-GP in doses from 2.5 to 10.0 mg kg− 1 body weight. Results: AgNP-GP nanoparticles were characterized through UV (405 nm), core size (20± 5 nm through TEM), hydrodynamic size (70– 80 nm), Zeta (ζ) potential (- 26 mV) using DLS and Powder X ray diffraction (PXRD) and EDS. PXRD showed pattern consistent with the Ag (1 1 1) peak. EC50 in Daphnia similis was 4.40 (3.59– 5.40) μg L− 1. In the zebrafish species, LC50 was 177 μg L− 1. Oral administration of AgNP-GP in Sprague Dawley rats for a period of 28 days revealed no adverse effects in doses of up to 10.0 mg kg− 1 b.w. in both male and female animals. Conclusion: The non-toxicity of AgNP-GP in rats offers a myriad of applications of AgNP-GP in health and hygiene for use as antibiotics, antimicrobial and antifungal agents.pt_BR
dc.format.extent7359-7376pt_BR
dc.identifier.citationMAZIERO, JOANA S.; THIPE, VELAPHI C.; ROGERO, SIZUE O.; CAVALCANTE, ADRIANA K.; DAMASCENO, KELME C.; ORMENIO, MATHEUS B.; MARTINI, GISELA A.; BATISTA, JORGE G.S.; VIVEIROS, WILLIAM; KATTI, KAVITA K.; KARIKACHERY, ALICE R.; MOHANDOSS, DARSHAKUMAR D.; DHURVAS, RASHMI D.; NAPPINNAI, MOHANAVELU; ROGERO, JOSE R.; LUGAO, ADEMAR B.; KATTI, KATTESH V. Species-specific in vitro and in vivo evaluation of toxicity of silver nanoparticles stabilized with gum arabic protein. <b>International Journal of Nanomedicine</b>, v. 15, p. 7359-7376, 2020. DOI: <a href="https://dx.doi.org/10.2147/IJN.S250467">10.2147/IJN.S250467</a>. Disponível em: http://200.136.52.105/handle/123456789/31613.
dc.identifier.doi10.2147/IJN.S250467pt_BR
dc.identifier.issn1178-2013pt_BR
dc.identifier.orcid0000-0002-1737-3191pt_BR
dc.identifier.orcid0000-0002-2517-7772pt_BR
dc.identifier.orcidhttps://orcid.org/0000-0002-1737-3191
dc.identifier.orcidhttps://orcid.org/0000-0002-2517-7772
dc.identifier.orcidhttps://orcid.org/0000-0003-3396-6277
dc.identifier.percentilfi77.78pt_BR
dc.identifier.percentilfiCiteScore89.83
dc.identifier.urihttp://200.136.52.105/handle/123456789/31613
dc.identifier.vol15pt_BR
dc.relation.ispartofInternational Journal of Nanomedicinept_BR
dc.rightsopenAccesspt_BR
dc.subjectnanoparticles
dc.subjectnanotechnology
dc.subjectfishes
dc.subjectantimitotic drugs
dc.subjecttoxicity
dc.subjectaquatic organisms
dc.subjectdaphnia
dc.subjectrats
dc.subjectgum acacia
dc.subjectin vitro
dc.subjectin vivo
dc.titleSpecies-specific in vitro and in vivo evaluation of toxicity of silver nanoparticles stabilized with gum arabic proteinpt_BR
dc.typeArtigo de periódicopt_BR
dspace.entity.typePublication
ipen.autorVELAPHI CLEMENT THIPE
ipen.autorKELME CARDOSO DAMASCENO
ipen.autorMATHEUS BEANI ORMENIO
ipen.autorJORGE GABRIEL DOS SANTOS BATISTA
ipen.autorADEMAR BENEVOLO LUGAO
ipen.autorJOSE ROBERTO ROGERO
ipen.autorGISELA DE ASSIS MARTINI
ipen.autorADRIANA KUCHINSKI CAVALCANTE
ipen.autorSIZUE OTA ROGERO
ipen.autorJOANA DA SILVA MAZIERO
ipen.codigoautor15459
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ipen.codigoautor14163
ipen.contributor.ipenauthorVELAPHI CLEMENT THIPE
ipen.contributor.ipenauthorKELME CARDOSO DAMASCENO
ipen.contributor.ipenauthorMATHEUS BEANI ORMENIO
ipen.contributor.ipenauthorJORGE GABRIEL DOS SANTOS BATISTA
ipen.contributor.ipenauthorADEMAR BENEVOLO LUGAO
ipen.contributor.ipenauthorJOSE ROBERTO ROGERO
ipen.contributor.ipenauthorGISELA DE ASSIS MARTINI
ipen.contributor.ipenauthorADRIANA KUCHINSKI CAVALCANTE
ipen.contributor.ipenauthorSIZUE OTA ROGERO
ipen.contributor.ipenauthorJOANA DA SILVA MAZIERO
ipen.date.recebimento20-12
ipen.identifier.fi6.400pt_BR
ipen.identifier.fiCiteScore9.2
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ipen.type.genreArtigo
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sigepi.autor.atividadeLUGAO, ADEMAR B.:339:740:Npt_BR
sigepi.autor.atividadeROGERO, JOSE R.:1118:740:Npt_BR
sigepi.autor.atividadeBATISTA, JORGE G.S.:10274:740:Npt_BR
sigepi.autor.atividadeMARTINI, GISELA A.:10185:-1:Npt_BR
sigepi.autor.atividadeORMENIO, MATHEUS B.:14332:810:Npt_BR
sigepi.autor.atividadeDAMASCENO, KELME C.:14173:-1:Npt_BR
sigepi.autor.atividadeCAVALCANTE, ADRIANA K.:12073:740:Npt_BR
sigepi.autor.atividadeROGERO, SIZUE O.:1266:740:Npt_BR
sigepi.autor.atividadeMAZIERO, JOANA S.:14163:-1:Spt_BR
sigepi.autor.atividadeTHIPE, VELAPHI C.:15459:-1:N
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