Methodological study of photobiomodulation anti-edematous effects on carrageenan-induced inflammation on zebrafish
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Lasers in Medical Science
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The anti-inflammatory action of Photobiomodulation (PBM) is one of its
most reproducible effects in vivo. New models to study PBMeffects and
to investigate light dosimetry may bring different perspectives on the
field. Based on the importance of establishing new experimental models
and the advantages of using zebrafish for screening new drugs with potential
anti-inflammatory effects the present investigation evaluated the
effect of PBM on carragenan-induced abdominal edema in zebrafish.
Thirty Danio rerio (± 1 g) zebrafish were randomly divided into five
aquariums with 0.25L of water to establish three treatments: T1 - Naive
non-treated; T2 – Saline 0.9%-injected; T3 - carrageenan-injected (CG)
3.5% in the coelomic cavity. T4 –injected (CG) 3.5% and treated with a
diode laser λ=808nm, 50mWand 0.5J T5 –same parameters with 1J and
T6 -1.5J. Abdominal volumes were measured by a caliper and the area
was calculated 4 hours post-indu ction. Four hours after edema induction
the results revealed an increase in the abdominal area of fish injected with
CG 3.5% compared to negative control. The animals injected with CG
and treated with PBM exhibited a reduction in abdominal edema in a
dose-dependent manner. The 0.5J energy did not present a significant
reduction compare do control (p=0.18), the 1J and 1.5J groups presented
a significant difference (p=0.027 and p=0.005 respectively). The 1.5J
group was not different from negative control (T2) p=0.37. The present
study identified that zebrafish may represent a good model to investigate
PBM effects and the response was dose dependent.
Como referenciar
NUNEZ, SILVIA C.; SILVA, IVES C. da; NASCIMENTO, JONATHAN S.; RIBEIRO, MARTHA S. Methodological study of photobiomodulation anti-edematous effects on carrageenan-induced inflammation on zebrafish. Lasers in Medical Science, v. 35, n. 1, p. 288-289, 2020. DOI: 10.1007/s10103-019-02900-7. Disponível em: http://repositorio.ipen.br/handle/123456789/31112. Acesso em: 20 Mar 2026.
Esta referência é gerada automaticamente de acordo com as normas do estilo IPEN/SP (ABNT NBR 6023) e recomenda-se uma verificação final e ajustes caso necessário.