Optimization and therapeutic effects of PDT mediated by ALA and MAL in the treatment of cutaneous malignant lesions: a comparative study
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Journal of Biophotonics
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5-aminolevulinic acid (ALA) and its methylated ester
(MAL) are the most common topical agents used in
photodynamic therapy (PDT) as precursors of the photosensitizer
protoporphyrin IX (PpIX). The induction of
newly PpIX depends on incubation time of each photosensitizer
in the tissue and the presence of high intralesional
porphyrin levels is an important parameter for the
PDT effectiveness. This study used laser-induced fluorescence
(LIF) spectroscopy to evaluate the optimum time
to light exposure of PDT mediated by ALA (20% w/w)
and MAL (10% w/w) to treat malignant lesions precursors
of cutaneous squamous cell carcinoma induced in
mice. The therapeutic effects obtained by optimized
ALA- and MAL-PDT were assessed 10 and 20 days after
treatments. Higher PpIX levels were evidenced in the lesions
photosensitized by ALA than MAL and according
to LIF measurements the PDT irradiation was performed,
respectively, at 300 and 330 minutes after ALA and MAL
incubation. Histopathological analysis evidenced necrosis
and epithelial atrophy after 10 days of PDT using both
prodrugs, as well as reepitelization and collagen deposition
at 20 days. Thus, despite the distinct concentration of
ALA and MAL used in the formulation of each photosensitizing
cream, PDT mediated by both photosensitizing
agents obtained similar therapeutic outcomes.
Como referenciar
LIMA, CASSIO A.; GOULART, VIVIANE P.; BECHARA, ETELVINO J.H.; CORREA, LUCIANA; ZEZELL, DENISE M. Optimization and therapeutic effects of PDT mediated by ALA and MAL in the treatment of cutaneous malignant lesions: a comparative study. Journal of Biophotonics, v. 9, n. 11-12, p. 1355-1361, 2016. DOI: 10.1002/jbio.201600164. Disponível em: http://repositorio.ipen.br/handle/123456789/27192. Acesso em: 30 Dec 2025.
Esta referência é gerada automaticamente de acordo com as normas do estilo IPEN/SP (ABNT NBR 6023) e recomenda-se uma verificação final e ajustes caso necessário.