Laminin-based skin substitutes in a burn animal model
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Tissue Engineering Part A
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TERMIS - AMERICAS CONFERENCE AND EXHIBITION
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Available treatments for skin regeneration are insufficient for
promoting healing. The current study has aimed to produce a cutaneous
substitute uniting mesenchymal stem cells, keratinocytes,
and a PDLLA biomaterial constructed by electrospinning to use in
nude mice. Six groups were tested: (1) only PDLLA; (2) only
PDLLA/Lam, a hydrolyzed scaffold with the binding of laminin;
(3) PDLLA with cells; (4) PDLLA/Lam with cells (n = 6/group) and
(5) animals injured without scaffolds (lesion control group) and (6)
healthy control group (n = 4/group). All the animals had 1 cm2 defect
performed on their backs, removing all the skin. The biomaterials(
or scaffolds) were implanted in the mice for up to 9 days.
Part of the defect was taken for histology and another for gene expression. Group 2 presented the best appearance with the softest
wound. Gene expression analysis showed a considerable increase
of TGFb1 expression, increased VEGF and balance of the BAX/
Bcl-2 ratio for the biomaterial groups when compared to the lesion
group. Histological analysis showed well-formed tissue in the
groups where the biomaterials and biomaterials plus cells were
used. In some animals, in which biomaterials and cells were used,
the epidermis was formed throughout the length of the wound. In
conclusion, these biomaterials are capable of providing support for
the growth of cells, indicating that they can be suitable biomaterials
for use in tissue engineering.
Como referenciar
STEFFENS, D.; MATHOR, M.B.; SOSTER, P.; VERGANI, G.; LUCO, D.P.; PRANKE, P. Laminin-based skin substitutes in a burn animal model. Tissue Engineering Part A, v. 22, p. S-80 - S-80, 2016. 1. Disponível em: http://repositorio.ipen.br/handle/123456789/27820. Acesso em: 27 Mar 2026.
Esta referência é gerada automaticamente de acordo com as normas do estilo IPEN/SP (ABNT NBR 6023) e recomenda-se uma verificação final e ajustes caso necessário.