Gamma irradiation of Toxoplasma gondii protein extract improve immune response and protection in mice models
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2018
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Biomedicine & Pharmacotherapy
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Gamma radiation induces protein changes that enhance immunogenicity for venoms, used in antivenin production.
Coccidian parasites exposed to gamma radiation elicit immune response with protection in mice and
man, but without studies on the effect of gamma radiation in soluble acellular extracts or isolated proteins.
Toxoplasmosis is a highly prevalent coccidian disease with only one vaccine for veterinary use but with remaining
tissue cysts. Total parasite extracts or recombinant proteins used as immunogen induce usually low
protection. Here, we study gamma radiation effect on T. gondii extracts proteins (STAG) and its induced immunity
in experimental mice models. By SDS-PAGE, protein degradation is seen at high radiation doses, but at
ideal dose (1500 Gy), there are preservation of the antigenicity and immunogenicity, detected by specific antibody
recognition or production after mice immunization. Immunization with STAG irradiated at 1500 Gy induced
significant protection in mice immunized and challenged with distinct T. gondii strains. In their blood,
higher levels of specific CD19+, CD3+CD4+ and CD3+CD8+ activated cells were found when compared to mice
immunized with STAG. Irradiated T. gondii tachyzoites extracts induce immune response and protection in mice
in addition, could be a feasible alternative for Toxoplasma vaccine.
Como referenciar
COSTA, ANDREA da; ZORGI, NAHIARA E.; NASCIMENTO, NANCI do; GALISTEO JUNIOR, ANDRES J.; ANDRADE JUNIOR, HEITOR F. de. Gamma irradiation of Toxoplasma gondii protein extract improve immune response and protection in mice models. Biomedicine & Pharmacotherapy, v. 106, p. 599-604, 2018. DOI: 10.1016/j.biopha.2018.06.155. Disponível em: http://repositorio.ipen.br/handle/123456789/29295. Acesso em: 14 Mar 2025.
Esta referência é gerada automaticamente de acordo com as normas do estilo IPEN/SP (ABNT NBR 6023) e recomenda-se uma verificação final e ajustes caso necessário.