NF-ĸΒ1 knockout reduces IL6 expression under hypoxia in renal cell carcinoma
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Cellular and Molecular Biology
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Renal cell carcinoma (RCC) is the most common adult renal epithelial cancer, accounting for more than 90%
of all renal neoplasms. Clear cell RCC (ccRCC) is the most common subtype of RCC. Most patients with
ccRCC have a mutation in the von Hippel-Lindau (VHL) tumor suppressor gene, which encodes a protein
that downregulates various intracellular proteins, including hypoxia-inducible factor (HIF). Many molecules
have been identified to be responsible for the aggressive phenotype of ccRCC, including the transcription
factor nuclear factor kappa B (NF-кB). The increase in NF-кB activity observed in RCC is correlated with an
increase in angiogenesis markers, such as interleukin 6 (IL-6). In recent years, several groups have demonstrated the functional role of NF-кB1 in RCC tumorigenicity. Herein, we used the CRISPR/Cas-9 technique
to obtain an NF-кB1 knockout-human renal adenocarcinoma cell line. Expression of IL-6 at the mRNA and
protein levels was analyzed under normoxia and hypoxia by real time-polymerase chain reaction and multiplex
assay, respectively. The CRISPR/Cas9 technique was effective in producing 786-0 knockout cells for NF-κB1
(p105/p50), as confirmed by western blot analysis. Suppression of p50 expression in 786-0 single guide RNA
(sg)1, 786-0 sg2 and 786-0 sg3 cells downregulated IL-6 mRNA and protein expression under normoxia and
hypoxia. The observed decrease in the differential expression of IL-6 in hypoxia/normoxia is suggestive of a
change in cellular responsiveness to hypoxia with respect to IL-6.
Como referenciar
TEIXEIRA, LUIZ F.S.; BELLINI, MARIA H. NF-ĸΒ1 knockout reduces IL6 expression under hypoxia in renal cell carcinoma. Cellular and Molecular Biology, v. 69, n. 6, p. 8-14, 2023. DOI: 10.14715/cmb/2023.69.6.2. Disponível em: http://repositorio.ipen.br/handle/123456789/34222. Acesso em: 20 Mar 2026.
Esta referência é gerada automaticamente de acordo com as normas do estilo IPEN/SP (ABNT NBR 6023) e recomenda-se uma verificação final e ajustes caso necessário.